Updated on 2024/01/31

写真a

 
chengzhu yin
 
Affiliation
Faculty of Medicine, Department of Physiology, Assistant Professor
Title
Assistant Professor
External link

Degree

  • 医学博士 ( 日本医科大学 )

Research Areas

  • Life Science / Neuroscience-general

Research History

Papers

  • Two Types of Cl Transporters Contribute to the Regulation of Intracellular Cl Concentrations in ON- and OFF-type Bipolar Cells in the Mouse Retina Reviewed

    Chengzhu Yin, Toshiyuki Ishii, Makoto Kaneda

    Neuroscience   440   267 - 276   2020.8

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    Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.neuroscience.2020.06.004

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  • Variation in the phenotype of photosensitive cells produced from human fibroblast cell lines Reviewed

    Toshiyuki Ishii, Chengzhu Yin, Yuko Seko, Akihiro Umezawa, Makoto Kaneda

    Journal of Nippon Medical School   85 ( 2 )   110 - 116   2018

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Medical Association of Nippon Medical School  

    Background: Photoreceptors differentiated from somatic cells are a useful tool for transplantation and drug screening. We previously showed that photosensitive cells are differentiated from human fibroblasts by direct reprogramming. In induced pluripotent stem (iPS) cells or embryonic stem (ES) cells, the properties of differentiated cells differ among the source of cell lines. However, whether or not the properties of the photosensitive cells produced by direct reprogramming are controlled by the origin of the cell line remains unknown. Methods: We compared the morphological and physiological properties of photosensitive cells induced by two fibroblast cell lines. Results: The differentiated cells had larger somas and more primary processes than the non-infected cells in both cell lines. The degree of morphological change was statistically different between the two cell lines. In addition, physiological responses to light differed between the two cell lines. An outward current (photoreceptor-like response) was observed in both cell lines, while an inward current (intrinsically photosensitive retinal ganglion cell-like response) was observed only in one cell line under light stimulation. Conclusions: These results suggest that photosensitive cells produced from different cell lines by direct reprogramming might express different phenotypes.

    DOI： 10.1272/jnms.2018_85-17

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  • Somatostatin Inhibition of GnRH Neuronal Activity and the Morphological Relationship between GnRH and Somatostatin Neurons in Rats Reviewed

    Makiko Koyama, Chengzhu Yin, Hirotaka Ishii, Yasuo Sakuma, Masakatsu Kato

    ENDOCRINOLOGY   153 ( 2 )   806 - 814   2012.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ENDOCRINE SOC  

    In rodents, GnRH neurons are diffusely distributed from the medial septum through to the medial preoptic area and control gonadal functions through the pituitary. The activity of GnRH neurons is regulated by a variety of bioactive substances, including the inhibitory peptide somatostatin. In the present study, we focused on somatostatin because intracerebroventricular injection of somatostatin inhibits the LH surge in rats and reduces LH secretion in ewes. Somatostatin also decreases GnRH release from rat hypothalamic slices. In mice, somatostatin is also thought to suppress GnRH neuronal activity through contact on the soma of GnRH neurons. However, similar data are missing in rats. Moreover, rat GnRH neurons receive only a few synaptic inputs. In this study, we assessed the morphological relationship between GnRH and somatostatin neurons. Confocal microscopy on the sections from the medial septum through medial preoptic area revealed about 35 close contacts per rat between the GnRH and somatostatin neuronal fibers in the organum vasculosum of the lamina terminalis region. No contact of somatostatin fibers on the GnRH neuronal somata was observed. Multicell RT-PCR for somatostatin receptor mRNA in rat GnRH neurons was also performed, which revealed moderate expression of somatostatin receptor subtypes 1-5. In addition, patch clamp experiments were carried out in acute slice preparations. Somatostatin suppressed neuronal firing in cells recorded in a cell-attached configuration and also induced whole-cell outward currents in GnRH neurons. These findings suggest that somatostatin directly inhibits the activity of rat GnRH neurons through volume transmission in the organum vasculosum of the lamina terminalis region. (Endocrinology 153: 806-814, 2012)

    DOI： 10.1210/en.2011-1374

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  • Voltage-gated Ca2+ channel mRNAs and T-type Ca2+ currents in rat gonadotropin-releasing hormone neurons Reviewed

    Nobuyuki Tanaka, Hirotaka Ishii, Chengzhu Yin, Makiko Koyama, Yasuo Sakuma, Masakatsu Kato

    JOURNAL OF PHYSIOLOGICAL SCIENCES   60 ( 3 )   195 - 204   2010.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER TOKYO  

    Gonadotropin-releasing hormone (GnRH) neurons play a pivotal role in the neuroendocrine regulation of reproduction. We have previously reported that rat GnRH neurons exhibit voltage-gated Ca2+ currents. In this study, oligo-cell RT-PCR was carried out to identify subtypes of the alpha(1) subunit of voltage-gated Ca2+ channels in adult rat GnRH neurons. GnRH neurons expressed mRNAs for all five types of voltage-gated Ca2+ channels. For T-type Ca2+ channels, alpha(1H) was dominantly expressed in GnRH neurons. Electrophysiological analysis in acute slice preparations revealed that GnRH neurons from adult rats exhibited T-type Ca2+ currents with fast inactivation kinetics (similar to 20 ms at -30 mV) and a time constant of recovery from inactivation of similar to 0.6 s. These results indicate that rat GnRH neurons express subtypes of the alpha(1) subunit for all five types of voltage-gated Ca2+ channel, and that alpha(1H) was the dominant subtype in T-type Ca2+ channels.

    DOI： 10.1007/s12576-010-0085-z

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  • Ca2+ Channels and Ca2+-Activated K+ Channels in Adult Rat Gonadotrophin-Releasing Hormone Neurones Reviewed

    M. Kato, N. Tanaka, H. Ishii, C. Yin, Y. Sakuma

    JOURNAL OF NEUROENDOCRINOLOGY   21 ( 4 )   312 - 315   2009.4

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    Language：English   Publisher：WILEY-BLACKWELL  

    Gonadotrophin-releasing hormone (GnRH) neurones represent the final output neurones in the neuroendocrine system for the control of reproduction. To understand the reproductive neuroendocrine system, an investigation of the intrinsic and extrinsic properties of GnRH neurones is essential. In this review, we focus on the intrinsic properties and summarise our recent findings of ion channels expressed in rat GnRH neurones. Rat GnRH neurones express all four types of high voltage-activated Ca(2+)channel (L, N, P/Q, R) and the low voltage-activated Ca2+ channel (T). GnRH neurones also express two types of Ca2+-activated K+ [K(Ca)] channel: the small conductance Ca2+-activated K+ (SK) channel and the large conductance Ca2+- and voltage-activated K+ (BK) channel. The activities of these Ca2+ and K(Ca) channels regulate cell excitability and cellular calcium homeostasis.

    DOI： 10.1111/j.1365-2826.2009.01849.x

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  • Cetrorelix, a Gonadotropin-Releasing Hormone Antagonist, Induces the Expression of Melatonin Receptor 1a in the Gonadotropin-Releasing Hormone Neuronal Cell Line GT1-7 Reviewed

    Hirotaka Ishii, Shun Sato, Chengzhu Yin, Yasuo Sakuma, Masakatsu Kato

    NEUROENDOCRINOLOGY   90 ( 3 )   251 - 259   2009

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：KARGER  

    Melatonin has been implicated in the control of the reproductive system, and the modulatory actions of melatonin on gonadotropin-releasing hormone (GnRH) neurons have been assumed to be indirectly mediated through afferent neurons. However, our previous studies demonstrate sexually dimorphic modulation of A-type gamma-aminobutyric acid (GABA) receptor (GABA(A)R) currents by melatonin in adult rat GnRH neurons and a preferential expression of melatonin 1a receptor (MT1) in male GnRH neurons. Using immortalized GnRH neurons (GT1-7 cells), the present study investigated the mechanism by which the expression of melatonin receptors is regulated in GnRH neurons. Like endogenous GnRH neurons, GT1-7 cells express both GnRH and GnRH receptor mRNAs, indicating that the cells have a self-stimulatory system. A 2-iodomelatonin binding assay and RT-PCR analysis demonstrated that the cells expressed neither MT1 nor MT2. However, treatment of GT1-7 cells with the GnRH antagonist cetrorelix significantly increased 2-iodomelatonin binding and induced a time- and concentration-dependent MT1 mRNA expression. The GABA(A)R currents were then measured using a perforated patch-clamp technique to examine whether the treatment with cetrorelix changed the responses to melatonin. Melatonin augmented the GABA(A)R currents in GT1-7 cells treated with 1 mu M cetrorelix for 24 h, while melatonin decreased the currents in the cells not treated with cetrorelix, probably via receptor-independent processes. The present results suggest that GnRH downregulates the expression of MT1 via an autocrine-paracrine mechanism in GT1-7 cells, and modifies the melatonin-induced modulation of GABA(A)R currents. These findings may provide one possible mechanism for the sexually dimorphic responses to melatonin in adult rat GnRH neurons. Copyright (C) 2009 S. Karger AG, Basel

    DOI： 10.1159/000231993

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  • Sexually Dimorphic Modulation of GABA(A) Receptor Currents by Melatonin in Rat Gonadotropin-Releasing Hormone Neurons Reviewed

    Shun Sato, Chengzhu Yin, Akira Teramot, Yasuo Sakuma, Masakatsu Kato

    JOURNAL OF PHYSIOLOGICAL SCIENCES   58 ( 5 )   317 - 322   2008.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER JAPAN KK  

    Gonadotropin-releasing hormone (GnRH) neurons represent the final output neurons in the central control of reproduction. gamma-Amino butyric acid (GABA), one of the major regulators of GnRH neurons, depolarizes GnRH neurons isolated from adult rats via GABA(A) receptors. The presence of GABA(A) receptors in GnRH neurons has also been demonstrated morphologically. Furthermore, the pineal hormone melatonin is involved in the regulation of reproductive function, including the timing of the luteinizing hormone surge. The suprachiasmatic nucleus and the GABAergic system in the medial preoptic area are considered as possible sites of the action of melatonin. Until now, however, a direct action of melatonin on GnRH neurons has not been reported. Therefore we examined the effect of melatonin on GABA(A) receptor currents in GnRH neurons isolated from GnRH-EGFP transgenic rats by means of perforated patchclamp experiments. The GABA(A) receptor currents were modulated by melatonin in a sex-specific manner. In GnRH neurons from adult males, melatonin augmented these currents in 67% of the neurons examined, but attenuated the currents in only 19% of them. These modulations were blocked by the melatonin receptor antagonist luzindole, suggesting an involvement of melatonin receptors. The modulation by melatonin was not observed in GnRH neurons isolated from infantile rats. These findings indicate that GABA affects the excitability of GnRH neurons in adult rats through GABAA receptors, and that melatonin modifies this excitability via melatonin receptors in a sex-specific manner.

    DOI： 10.2170/physiolsci.RP006208

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  • Activation of A-type gamma-amino butyric acid receptors excites gonadotrophin-releasing hormone neurones isolated from adult rats Reviewed

    C. Yin, H. Ishii, N. Tanaka, Y. Sakuma, M. Kato

    JOURNAL OF NEUROENDOCRINOLOGY   20 ( 5 )   566 - 575   2008.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：BLACKWELL PUBLISHING  

    Gonadotrophin-releasing hormone (GnRH) neurones represent the final output neurones in the neuroendocrine control of reproduction, and gamma-amino butyric acid (GABA) is one of the major players in the regulation of GnRH neurones. GABA inhibits a large proportion of brain neurones in adult animals by acting on A-type GABA receptors (GABA(A)Rs). Two contradictory reports on the action of GABA in the GnRH neurones of adult mice have been published. DeFazio et al. (Mol Endocrinol 2002; 16: 2872) demonstrated that activation of GABA(A)Rs excites the GnRH neurones of adult mice, whereas Han et al. (Endocrinology 2002; 143: 1459) showed that the response to GABA on GnRH neurones switches from depolarisation to hyperpolarisation around puberty in female mice. Therefore, we examined the reversal potential of GABA(A)R currents by means of perforated patch-clamp recording with gramicidin in overnight-cultured GnRH neurones isolated from adult GnRH-enhanced green fluorescent protein transgenic rats. The reversal potential was -26 +/- 1.4 mV (mean +/- SEM, n = 42) in GnRH neurones, whereas it was -57 +/- 2.7 mV (n = 34) in unidentified neurones, and GABA depolarised the GnRH neurones in current-clamp condition. The GABA(A)R currents in rat GnRH neurones were augmented by neurosteroids, allopregnanolone and 3 alpha,21-dihydroxy-5 alpha-pregnan-20-one, at submicromolar concentrations. In addition, the expression patterns of GABA(A)R subunit mRNAs were determined by multi-cell reverse transcription-polymerase chain reaction, which revealed that the alpha 2, beta 3, gamma 1 and gamma 2 subunits were dominant and the alpha 6 and gamma 3 subunits were negative in rat GnRH neurones. These results indicate that GABA(A)Rs in the soma of rat GnRH neurones are comprised mainly of alpha 2, beta 3 and gamma 1 or gamma 2 subunits and that they are sensitive to neurosteroids; moreover, they suggest that activation of these receptors depolarises GnRH neurones. Thus, GABA and neurosteroids influence the electrical activity of GnRH neurones.

    DOI： 10.1111/j.1365-2826.2008.01697.x

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Misc.

  • ラットGnRHニューロン及びGT1‐7細胞株に発現するGABA受容体の発現解析

    石井寛高, 尹成珠, 梶尾円香, 棟朝亜理紗, 加藤昌克, 宮本武典, 佐久間康夫

    日本内分泌学会雑誌   88 ( 2 )   639   2012.9

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    Language：Japanese  

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  • ラットGnRHニューロンの緩徐後過分極電流はSKチャネルとKCNQチャネルの活性化により惹起される

    尹成珠, 石井寛高, 佐久間康夫, 加藤昌克

    日本内分泌学会雑誌   88 ( 2 )   639   2012.9

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  • ガラニン受容体シグナリングによるキスペプチン誘導性黄体形成ホルモン分泌の調節

    家田菜穂子, 上野山賀久, 山本恵理, 岡良隆, 杉原稔, 小野幸輝, 諏訪牧子, 石井寛高, 加藤昌克, 尹成珠, 佐久間康夫, 前多敬一郎, 束村博子

    日本内分泌学会雑誌   88 ( 1 )   351   2012.4

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  • ラットGnRHニューロンに発現するGABA受容体の発現解析

    石井寛高, 尹成珠, 加藤昌克, 佐久間康夫

    日本下垂体研究会学術集会プログラム・講演要旨集   27th   45   2012

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  • ラットGnRHニューロンに発現するA型GABA受容体(GABAAR)のサブユニット構成

    尹成珠, 石井寛高, 加藤昌克, 佐久間康夫

    日本内分泌学会雑誌   86 ( 2 )   347   2010.9

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  • Neurosteroids augment A-type gamma-aminobutyric acid receptor currents in rat gonadotropin-releasing hormone neurons

    Chengzhu Yin, Hirotaka Ishii, Nobuyuki Tanaka, Masakatsu Kato, Yasuo Sakuma

    JOURNAL OF PHYSIOLOGICAL SCIENCES   60   S48 - S48   2010

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    Language：English   Publishing type：Research paper, summary (international conference)   Publisher：SPRINGER TOKYO  

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  • NEUROSTEROIDS AUGMENT A TYPE gamma-AMINOBUTYRIC ACID RECEPTOR (GABA(A)) CURRENTS IN RAT GnRH NEURONS

    Chengzhu Yin, Hirotaka Ishii, Nobuyuki Tanaka, Yasuo Sakuma, Masakatsu Kato

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   334 - 334   2009

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    Language：English   Publishing type：Research paper, summary (international conference)   Publisher：SPRINGER TOKYO  

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  • T-TYPE Ca2+ CHANNELS IN ADULT RAT GONADOTROPIN-RELEASING HORMONE (GnRH) NEURONS

    Nobuyuki Tanaka, Hirotaka Ishii, Chengzhu Yin, Yasuo Sakuma, Masakatsu Kato

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   334 - 334   2009

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    Language：English   Publishing type：Research paper, summary (international conference)   Publisher：SPRINGER TOKYO  

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  • ラットGnRHニューロンにおける電位依存性カルシウムチャネルの発現の発達段階による差異

    田中伸幸, 石井寛高, 尹成珠, 加藤昌克, 佐久間康夫

    日本内分泌学会雑誌   84 ( 2 )   539 - 539   2008.9

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  • GnRHアンタゴニストのcetrorelixは,GT1‐7細胞のメラトニン受容体1aの発現を誘導する。

    石井寛高, 尹成珠, 加藤昌克, 佐久間康夫

    日本内分泌学会雑誌   84 ( 2 )   522 - 522   2008.9

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  • GnRHアンタゴニストのcetrorelixは,GT1‐7細胞のメラトニン受容体1aの発現を誘導する。

    石井寛高, 尹成珠, 加藤昌克, 佐久間康夫

    日本神経内分泌学会プログラム及び抄録集   35th   62   2008

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  • ラットGnRHニューロンにおける電位依存性カルシウムチャネルの発現の発達段階による差異

    田中伸幸, 石井寛高, 尹成珠, 加藤昌克, 佐久間康夫

    日本神経内分泌学会プログラム及び抄録集   35th   79   2008

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  • Presence of gabazine-resistant GABA(A) receptor currents and their modulation by neurosteroids in rat GnRH (LHRH) neurons

    Chengzhu Yin, Nobuyuki Tanaka, Hirotaka Ishii, Masakatsu Kato, Yasuso Sakuma

    NEUROSCIENCE RESEARCH   58   S66 - S66   2007

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    Language：English   Publishing type：Research paper, summary (international conference)   Publisher：ELSEVIER IRELAND LTD  

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  • GABA increased the intracellular calcium concentration of GnRH neurons isolated from adult GnRH-EGFP transgenic rats

    Nobuyuki Tanaka, Miho Watanabe, Yin Chengzhu, Yasuo Sakuma, Masakatsu Kato

    FRONTIERS IN NEUROENDOCRINOLOGY   27 ( 1 )   76 - 77   2006.5

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    Language：English   Publishing type：Research paper, summary (international conference)   Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE  

    DOI： 10.1016/j.yfrne.2006.03.160

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  • GABA depolarizes GnRH neurons isolated from adult GnRH-EGFP transgenic rats

    YIN C

    Jpn Soc Neurosci Progr   29   G125   2006

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Presentations

  • Slow afterhyperpolarization (sAHP) current in rat GnRH neurons is carried through SK and KCNQ channels

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  • Effects of anion permeability of GABAA and GABAC receptors on surround response polarity in bipolar cells of the mouse retina

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Research Projects

  • Mechanism analysis of mouse bipolar cell surround response polarity reversal

    Grant number：16K11333  2016.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    yin chengzhu, kaneda makoto

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    Grant amount：\3120000 （ Direct Cost: \2400000 、 Indirect Cost：\720000 ）

    Surround responses of bipolar cells are explained by the hypothesis that the differences of the reversal potential of GABA-mediated chloride currents reverse the polarity between ON and OFF bipolar cells.In this study, we will investigate the distribution pattern of Cl inon transpoter in ON and OFF bipolar cells, and clarify the mechanism of surround response polarity reversal.
    The difference between NKCC1 and KCC2 distribution was confirmed in ON and OFF bipolar cells using electrophysiological, immunohistochemical method and fluorescence in situ hybridization method. As a result, the expression level of NKCC1 was high in ON bipolar cells, low in OFF bipolar. KCC2 showed almost the same expression level in both ON and OFF bipolar cells. rom these results, it is the intracellular Cl ion concentration that reverses the retina bipolar cell surround response polarity, and the concentration difference is caused by difference of Cl ion transpoer NKCC1 and KCC2 distribution.

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