記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: Children who survive traffic accidents, and their parents, may develop post-traumatic stress disorder (PTSD) or related symptoms (depression or anxiety), which can hinder the children's development and the parents' ability to provide effective care. In Japan, the PTSD incidence rate following traffic accidents and its related factors remain unclarified. METHOD: Participants were 79 children and 104 parents. The children were aged 3-18 years when injured. From August-December 2015, participants completed a self-reported questionnaire survey that comprised the 15-item Post-traumatic Stress Symptoms for Children and the Japanese version of the Impact of Event Scale-Revised. The children's Injury Severity Score (ISS) was also obtained from their medical records. Correlations, analyses of variance, and multiple regression analyses were conducted. RESULTS: Among the children and the parents, 10.2% and 22.1% were deemed to be at high risk of PTSD, respectively. Their stress scores were significantly positively correlated with each other and negatively correlated with children's age at the time of the accident. Parents who witnessed their children's accidents and those whose children were hospitalized were more stressed. Neither the children's nor the parents' risk for PTSD was associated with the ISS and the amount of time since the accident. CONCLUSIONS: A system that simultaneously works with children and parents, to support both parties' psychological recovery is required. To ensure psychological care post-injury, it is necessary to evaluate PTSD risk, regardless of injury severity. Implementing preventive and early interventions can prove more valuable than awaiting natural recovery.

DOI： 10.1272/jnms.JNMS.2022_89-105

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Clear cell sarcoma of the kidney (CCSK) is a rare aggressive pediatric renal tumor. Intratumoral hemorrhage and tumor rupture are considered as oncologic emergencies, requiring a quick and appropriate response. Herein, we report the case of an 11-year-old boy, who visited our hospital with abdominal distension that started a month earlier. Computed tomography (CT) revealed a tumor in the left kidney (size: 200 mm), and a diagnosis of CCSK was established with a biopsy. Chemotherapy was initiated to shrink the large and densely vascularized tumor before surgical removal. Two days after initiating chemotherapy, the patient developed abdominal and back pain, anemia, and hypotension. The CT scan showed intratumoral bleeding. Emergency transcatheter arterial embolization (TAE) was performed to control the bleeding. Three tumor feeding vessels were identified: an ascending branch from the celiac artery, an intermediate branch from the left renal artery, and a descending branch from the inferior mesenteric artery, of which the intermediate and descending branches were large and bleeding profusely; therefore, the intermediate branch was injected with ethanol and the descending branch was subjected to gel-foam embolization. Chemotherapy was resumed, and the patient's condition stabilized gradually. The tumor began to shrink, and subsequent chemotherapy progressed well. In week 12 of chemotherapy, the patient underwent tumor resection and left nephrectomy. Postoperative chemotherapy was completed without complications, and there was no recurrence during a 6-year follow-up. Therefore, TAE can effectively control intratumoral bleeding in pediatric solid tumors and consequently prevent an unnecessary high-risk open surgical procedure.

DOI： 10.1272/jnms.JNMS.2022_89-108

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1038/s41375-021-01171-y

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Not available.

DOI： 10.3324/haematol.2020.266320

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

PURPOSE OF REVIEW: Coronavirus disease 2019 (COVID-19), a respiratory infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 virus), is a pandemic in over 120 countries worldwide. Risk factors for severe COVID-19 include older age, ethnicity, sex, comorbidities, and living conditions. Although asthmatics and those with allergies are susceptible to more severe outcomes to viral infections, interestingly, asthma has not been reported to be a major comorbidity of COVID-19. However, there are some conflicting reports on the impact of asthma on COVID-19. The underlying immunological and molecular mechanisms may explain at least in part these observations. Furthermore, environmental factors like air pollution that have detrimental effects on asthma and respiratory illnesses also have an impact on COVID-19. RECENT FINDINGS: Angiotensin-converting enzyme 2 (ACE2) is the receptor for the attachment and entry of SARS-CoV-2 into the host cells that is upregulated by Th1-mediated responses. In asthmatics, ACE2 gene expression is generally reduced and recent studies have shown a negative correlation between the levels of Th2 cytokines including IL-4, IL-5, and IL-13 in airway epithelial cells and other type 2 biomarkers with ACE2 expression. This may explain in part the potential protective role of asthma on COVID-19. Here, we review the relation of respiratory viral illnesses and asthma, the immune-molecular mechanisms of SARS-CoV-2 infection, the impact of asthma on COVID-19 and that of SARS-CoV-2 on asthma and allergic rhinitis, and the impact of environmental factors like air pollution on COVID-19. SUMMARY: Expression of ACE2 in airway epithelial cells in SARS-COV-2 is influenced by inflammatory profile. Respiratory allergic diseases like asthma appear to have a protective effect against SARS-COV-2 infection. However, the clinical association between asthma and SARS-COV-2 is not fully established and the underlying immune-molecular mechanisms may explain these observations.

DOI： 10.1097/ACI.0000000000000699

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記述言語：日本語   出版者・発行元：金原出版(株)  

＜文献概要＞学童期の貧血は小児でたびたび遭遇する鉄欠乏性貧血の頻度は低下する.本稿ではスポーツやピロリ菌が関与している鉄欠乏性貧血や診断・鑑別に苦慮する骨髄異形成症候群・先天性骨髄不全症などの希少疾患,慢性炎症性疾患,消化管の潰瘍や憩室,胃炎などの出血を伴う疾患等,学童期に貧血をきたす疾患について概説する.急性出血や悪性腫瘍も含めて,注意深く診断・治療する必要のある疾患が多く存在することを認識して,緊張感をもって診療することが大切である.

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記述言語：日本語   出版者・発行元：(株)南山堂  

＜Key Points＞◎鉄欠乏性貧血は小児では思春期と乳児期後期に頻度が高く、4〜5ヵ月の早産児でもみられる。◎乳児期の鉄欠乏性貧血は、ほとんどが母乳栄養児である。◎患者が規則正しく鉄剤を服用していない可能性が意外に多い。◎ヘリコバクター・ピロリ感染症の家族歴を確認する。◎鉄剤不応性や再燃をくり返す症例に遭遇するとき、患児の栄養状態、そして鉄剤の使用方法などを熟知しておくことが治療のキーポイントとなる。(著者抄録)

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記述言語：日本語   出版者・発行元：日本医科大学医学会  

15歳男児。突然の激しい腹痛を主訴に前医を受診し、急性腹症として当院へ紹介となった。腹部造影CT所見より、回盲部に同心円状構造を認め、腸重積症と診断された。治療としてガストログラフィンを用いた高圧浣腸で整復が試みられたが困難で、緊急手術が施行された。術中所見では回盲部内に硬い腫瘤が触知され、用手的整復は難しく、腫瘤を含む回盲部が切除された。その結果、病理検査でMurphy分類stage IIの回盲部悪性リンパ腫と確定診断された。術後は化学療法が4ヵ月行われたが、治療終了後1年経過現在、寛解を維持している。

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J04260&link_issn=&doc_id=20200710390005&doc_link_id=10.1272%2Fmanms.16.155&url=https%3A%2F%2Fdoi.org%2F10.1272%2Fmanms.16.155&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

記述言語：英語   掲載種別：研究論文（学術雑誌）  

Hemophagocytic lymphohistiocytosis (HLH) associated with Epstein-Barr virus (EBV) infection ranges from self-limiting to severe/aggressive or fatal. We report the case of a 4-year-old boy with EBV-HLH with persistent fever, severe pancytopenia, hypertriglyceridemia, and hypofibrinogenemia. The levels of plasma cytokines and chemokines were measured using a Bio-Plex system on Days 1, 2, 3, 4, 5, and 8 following hospital admission. The administration of steroid and high-dose intravenous immunoglobulin (1 g/kg) did not alleviate the fever or reduce cytokine production; however, following etoposide (a known antineoplastic agent) administration, the fever decreased immediately, and the patient' s general condition improved. The present study revealed that IL-6, IL-10, IL-8, MCP-1, IFN-γ, and TNF-α were suppressed by etoposide administration. In particular, production of IFN-γ sharply declined from 1,104.1 pg/mL to 101.5 pg/mL, and the IL-6 level also decreased from 229.8 pg/mL to 11.0 pg/mL the day after the initial etoposide administration. Subsequently, there was no recurrence of symptoms with dexamethasone, etoposide, and cyclosporine A treatment. The present case demonstrates that early etoposide administration is critical for successful treatment and indicates that etoposide exhibits a prompt inhibitory effect on cytokine production.

DOI： 10.1272/jnms.JNMS.2020_87-307

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記述言語：日本語   出版者・発行元：(株)診断と治療社  

●学童・思春期はスポーツ活動に心身を打ち込む時期であり、スポーツ貧血の存在を認識することが大切である。●スポーツ貧血のおもな病態は鉄欠乏であり、運動の種類や練習内容、競技環境、食生活にも目配せしながら治療・指導を進めていくことが大切である。●鉄剤に反応しない貧血の中にヘリコバクター・ピロリ(Helicobacter pylori)感染症や亜鉛欠乏による貧血もあるので注意したい。●最近話題となっている鉄の代謝調節ホルモンであるヘプシジンや不適切な鉄剤使用も含めて概説した。(著者抄録)

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J00642&link_issn=&doc_id=20200117220007&doc_link_id=%2Fae4shond%2F2020%2F008302%2F008%2F0183-0189%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fae4shond%2F2020%2F008302%2F008%2F0183-0189%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：英語   出版者・発行元：WILEY  

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記述言語：英語   出版者・発行元：AMER SOC HEMATOLOGY  

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DOI： 10.1182/blood-2019-125465

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/ped.14062

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

BACKGROUND: Therapeutic outcomes for childhood malignancy have dramatically improved. However, secondary malignancies are a major concern, as they greatly affect the quality of life of survivors. This retrospective study evaluated the cumulative incidence, clinical features, and outcomes of secondary malignancies at Nippon Medical School Hospital. METHODS: We examined data from 275 cases of primary childhood malignancy diagnosed between 1980 and 2014. Information regarding treatment of the primary malignancy, including irradiation dose, site, and cumulative dose of anticancer drugs, was assessed. We also collected data on secondary malignancy, including patient sex, age at diagnosis, malignancy site, time from primary to secondary malignancy, and outcomes. RESULTS: Secondary malignancies developed in 11 patients and included acute myeloid leukemia (AML) (4), meningioma (4), Ewing sarcoma (1), germ cell tumor (1), and malignant parotid gland tumor (1). The primary malignancies included acute lymphoblastic leukemia (ALL) (9), non-Hodgkin lymphoma (1) and brain tumor (1). In 7 of the 9 ALL patients, chemoradiotherapy was the primary treatment. The meningiomas and 1 solid tumor developed within the radiation field. All AMLs and meningiomas developed within 5 years and after 20 years, respectively, of the primary diagnosis. The 10- and 20-year cumulative incidence rates for secondary malignancy in our hospital were 1.9% and 5.8%, respectively. CONCLUSIONS: Our results revealed that the type of secondary malignancy depends on the interval after the end of treatment for primary malignancy. Meningioma, notably, develops many years after completion of primary malignancy treatment. Early detection during long-term follow-up is therefore essential.

DOI： 10.1272/jnms.JNMS.2018_86-401

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Fusion genes involving MEF2D have recently been identified in precursor B-cell acute lymphoblastic leukemia, mutually exclusive of the common risk stratifying genetic abnormalities, although their true incidence and associated clinical characteristics remain unknown. We identified 16 cases of acute lymphoblastic leukemia and 1 of lymphoma harboring MEF2D fusions, including MEF2D-BCL9 (n=10), MEF2D-HNRNPUL1 (n=6), and one novel MEF2D-HNRNPH1 fusion. The incidence of MEF2D fusions overall was 2.4% among consecutive precursor B-cell acute lymphoblastic leukemia patients enrolled onto a single clinical trial. They frequently showed a cytoplasmic μ chain-positive pre-B immunophenotype, and often expressed an aberrant CD5 antigen. Besides up- and down-regulation of HDAC9 and MEF2C, elevated GATA3 expression was also a characteristic feature of MEF2D fusion-positive patients. Mutations of PHF6, recurrent in T-cell acute lymphoblastic leukemia, also showed an unexpectedly high frequency (50%) in these patients. MEF2D fusion-positive patients were older (median age 9 years) with elevated WBC counts (median: 27,300/ml) at presentation and, as a result, were mostly classified as NCI high risk. Although they responded well to steroid treatment, MEF2D fusion-positive patients showed a significantly worse outcome, with 53.3% relapse and subsequent death. Stem cell transplantation was ineffective as salvage therapy. Interestingly, relapse was frequently associated with the presence of CDKN2A/CDKN2B gene deletions. Our observations indicate that MEF2D fusions comprise a distinct subgroup of precursor B-cell acute lymphoblastic leukemia with a characteristic immunophenotype and gene expression signature, associated with distinct clinical features.

DOI： 10.3324/haematol.2017.186320

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記述言語：日本語   出版者・発行元：(株)東京医学社  

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記述言語：英語   出版者・発行元：AMER SOC HEMATOLOGY  

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DOI： 10.1182/blood-2018-99-114290

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記述言語：日本語   出版者・発行元：(株)日本小児医事出版社  

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2018&ichushi_jid=J00643&link_issn=&doc_id=20180427030039&doc_link_id=%2Fag1snrsd%2F2018%2F007105%2F039%2F0841-0846%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fag1snrsd%2F2018%2F007105%2F039%2F0841-0846%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：日本語   出版者・発行元：金原出版(株)  

＜文献概要＞・小球性貧血の大部分は鉄欠乏性貧血で,思春期特有の着目点があり,そこにサラセミアや炎症性の小球性貧血が紛れ込んでくる.・多くの若者がスポーツ活動に心身を打ち込む時期でもある.スポーツ貧血の存在を認識し,関与している種々の要素を理解することができればきめ細かい対応が可能になる.・慢性炎症の小球性貧血では血清鉄は低下するが総鉄結合能の上昇はなく,血清フェリチンは正常または上昇する.・RBC 500万以上,Mentzer index(MCV/RBC×106)が13以下なら,鑑別に注意すべきサラセミアの可能性が高い.・鉄剤不応性や再燃を繰り返す症例に遭遇するとき,患児の栄養状態,そして鉄剤の使用方法などを熟知しておくことが治療のキーポイントとなる.

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記述言語：英語   出版者・発行元：WILEY  

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記述言語：日本語   出版者・発行元：(株)診断と治療社  

小球性貧血の大部分は鉄欠乏性貧血で、乳児、思春期、アスリートなどそれぞれに着目点があり、そこにサラセミアや炎症性の小球性貧血が紛れ込んでくる。慢性炎症の小球性貧血では血清鉄は低下するが総鉄結合能の上昇はなく、血清フェリチンは正常または上昇する。RBC 500万以上、Mentzer index(MCV/RBC)が13以下なら、サラセミアの可能性が高い。最近話題となっているHelicobacter pylori感染症や鉄の代謝調節ホルモンであるヘプシジンも含めて概説した。(著者抄録)

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2017&ichushi_jid=J00642&link_issn=&doc_id=20170925150003&doc_link_id=%2Fae4shond%2F2017%2F008010%2F004%2F1163-1168%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fae4shond%2F2017%2F008010%2F004%2F1163-1168%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：日本語   出版者・発行元：(株)東京医学社  

＜Key Points＞(1)離乳食の進まない完全母乳栄養児は鉄欠乏性貧血ハイリスクとの認識をもって診察する。(2)思春期の鉄欠乏性貧血は生活習慣の指導も大切である。(3)ビタミンB12欠乏は貧血より先に神経症状が進行することがあり、過分葉好中球の存在に注意する。(4)経腸栄養中の亜鉛投与により銅欠乏が起こる可能性がある。(著者抄録)

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記述言語：日本語   出版者・発行元：(一社)日本形成外科学会  

15歳女。5年前に鼻前底部の横紋筋肉腫を発症し、3回の手術に加え、化学療法、放射線療法を施行されたが再発を繰り返した。腫瘍は硬口蓋から中咽頭にかけて進展・増大し、気道狭窄をきたしたが、気管切開による延命は本人・家族とも望まず、腫瘍の縮小を目的に当科受診した。初診時、出血を伴う弾性硬の腫瘍が鼻腔から前方に突出しており、体表外の腫瘍は7×8×6cm大であった。手術による腫瘍縮小は多量の出血が予想され、また本人・家族とも全身麻酔下の手術は望んでいなかったため、Mohsペーストによる腫瘍の減量を行った。結果、1ヵ月で腫瘍の高さが半分になり、2ヵ月後には更に減量されて気道狭窄が改善し、経口摂取が可能となった。

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER JAPAN KK  

Residual disease (RD) after induction chemotherapy may predict clinical outcome in acute myeloid leukemia (AML). In the present study, we investigated the prognostic significance of RD detected by multidimensional flow cytometry (MDF) among 34 children treated for AML in a clinical trial (JPLSG AML-05) in Japan. Bone marrow samples were analyzed at the points of the end of the first induction course (BMA-1) and second induction course (BMA-2) by MDF. RD was evaluated by detecting the immature cells showing abnormal antigen expression pattern; CD34(+), CD15(+), CD7(+). Thirteen (39.4 %) of 34 patients at BMA-1 and 8 (27.6 %) of 34 at BMA-2 had RD levels >= 0.1 %. There was no significant difference in 3y-EFS and 3y-OS between patients with RD levels >= 0.1 % and those with RD levels <0.1 % (53.8 versus 70.0 %, P = 0.30 and 50.0 versus 66.7 %, P = 0.27, respectively). However, IR cytogenetics and negative FLT3-ITD patients with RD levels >= 0.1 % exhibited significantly lower 3y-EFS and 3y-OS than those with RD levels <0.1 % (33.3 versus 83.3 %, P = 0.02 and 20.0 versus 76.9 %, P = 0.005, respectively). Our study suggests that RD shows prognostic relevance in pediatric patients with IR cytogenetics and negative FLT3-ITD AML.

DOI： 10.1007/s12185-016-1937-y

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記述言語：日本語   出版者・発行元：日本医科大学医学会  

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2015&ichushi_jid=J04260&link_issn=&doc_id=20151207410002&doc_link_id=%2Fcw1nimed%2F2015%2F001104%2F003%2F0181-0186%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcw1nimed%2F2015%2F001104%2F003%2F0181-0186%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：日本語   出版者・発行元：(株)東京医学社  

＜Key Points＞(1)リンパ節を触知すればそれがただちに病的あるいは治療を必要とするリンパ節腫大であるとは限らない。(2)皮膚病変、口腔内病変、齲歯にも注意を払う。(3)悪性疾患を常に念頭において診察する。(4)針生検は極力避けるべきである。(著者抄録)

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記述言語：日本語   出版者・発行元：金原出版(株)  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL  

Osteoblastic cells are a key component of the bone marrow (BM) stem cell niche and help regulate hematopoietic stem cells (HSCs). We have previously demonstrated that adipose-derived stromal cells (ADSCs) can differentiate into both osteogenic and chondrogenic cells in vitro. The current study examined whether the anatomical architecture of the BM could be regenerated in vivo by using ADSCs cultured on a hydroxyapatite (HA) scaffold. ADSCs from GFP transgenic mice were cultured in vitro on an HA scaffold. The scaffold with the attached cells was implanted subcutaneously onto the backs of C57/BL6 (Ly5.2) recipient mice. Lineage-negative (Lin-) Ly5.1 BM cells transduced with a lentiviral vector containing the luciferase (Luc) gene were intravenously administered to the recipient mice after lethal irradiation. Eight weeks after BM transplantation, the scaffolds were removed from the first recipient mice and subcutaneously implanted into lethally irradiated second recipient mice. The biodistribution and kinetics of Luc(+) Ly5.1 cells were monitored by bioluminescence imaging and flow cytometry. Luc(+) hematopoietic cells were present in the scaffolds of the secondary implanted mice for at least 8 months. Subcutaneous injection of G-CSF resulted in wide distribution of bioluminescence signals from the original scaffolds to the whole body. Therefore, BM regenerated using ADSCs grown on an HA scaffold can support HSC populations in vivo, suggesting that a functional BM niche is reconstituted. These results may have a significant impact on the development of therapeutic strategies for various hematopoietic diseases.

DOI： 10.1002/cbin.10254

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MEDICAL ASSOC NIPPON MEDICAL SCH  

Gaucher disease is an autosomal recessively inherited lysosomal storage disease in which a deficiency of glucocerebrosidase is associated with the accumulation of glucocerebroside in reticuloendothelial cells. Clinically, 3 types of Gaucher disease have been defined on the basis of the presence or absence of neurological symptoms. The frequency of gallbladder involvement is reportedly greater in patients with type 1 Gaucher disease than in healthy persons. We report a case of recurrent cholelithiasis and liver failure in a patient with type 2 Gaucher disease who showed severe progressive neurological involvement.

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記述言語：英語   出版者・発行元：WILEY-BLACKWELL  

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記述言語：日本語   出版者・発行元：金原出版(株)  

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

An 11-year-old boy was experienced severe life-threatening hemorrhage from a branch of the superior mesenteric artery (SMA) after acute lymphoblastic leukemia induction therapy. The patient had a history of attention deficit hyperactivity disorder (ADHD), diagnosed at 3 years of age. Subsequent to discontinuing his psychotropic medication, the patient's mental status deteriorated and treatment with midazolam for 3 weeks was necessary to allow the completion of the leukemia induction regimen. On day 51, although there was no indication of thrombocytopenia or a coagulation disorder, the patient began to hemorrhage suddenly from anal with resulting hypovolemic shock, and large-volume blood transfusion was initiated. Although upper and lower endoscopy failed to determine the location of the hemorrhage, angiography enabled us to determine that it was a branch of the SMA (the middle colic artery #6), and selective arterial embolization was used to arrest the bleeding. There could have been underlying causes, such as, a probable malformation or aneurysm in that area, although there was no indication before or after the event. This is a rare case of arterial hemorrhage from a branch of the SMA that occurred in a pediatric patient idiopathically during the induction therapy of leukemia. Pediatrics International © 2013 Japan Pediatric Society.

DOI： 10.1111/j.1442-200X.2012.03682.x

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MEDICAL ASSOC NIPPON MEDICAL SCH  

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare, acquired clonal disorder characterized by chronic complement-mediated hemolysis. The humanized anti-05 antibody eculizumab binds to the C5 protein and suppresses hemolysis by inhibiting C5b-9 generation. Here, we report on a 27-year-old woman who was found to have PNH in 1997 (at 13 years of age), without subsequent transfusions, thrombosis, or renal disorder. She had been experiencing frequent malaise and fatigue and was sometimes unable to participate in social activities. She had also experienced repeated hemolytic episodes due to infection, and the hemoglobin level had decreased from 7.0 to 5.0 g/dL several times since February 2010. Red blood cell transfusion was necessary, and 6 months later, treatment with eculizumab was started. The hemoglobin level stabilized, and the patient became transfusion-independent. Furthermore, the patient showed significant improvements in fatigue scale scores and quality of life. Six months after the start of eculizumab therapy, the percentage of PNH-type red blood cells was found to have increased from 82.0% (1.95 x 10(12) cells/L) to 89.1% (2.78 x 10(12) cells/L). Furthermore, during treatment with eculizumab, intravascular hemolysis occurred due to a viral infection accompanied by a high fever. We also observed a persistent elevation in reticulocytes and total bilirubin levels, as well as a persistent reduction in haptoglobin levels. Extravascular hemolytic findings were also observed. Because treatment with eculizumab was started at a young age (27 years) and will be continued for many years, careful observation of the patient is required. (J Nippon Med Sch 2013; 80: 155-159)

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記述言語：日本語   出版者・発行元：(株)東京医学社  

＜Key Points＞(1)鉄欠乏性貧血の前の段階の鉄欠乏症から発達に影響を与える報告がある。(2)早産児は鉄欠乏性貧血を起こしやすい。(3)健診のときには貧血は意識して診察しないと発見はむずかしい。(4)近年、母乳栄養の割合が増加しており、月齢が進んでもその割合が高くなってきている。健診では完全母乳栄養児はハイリスクとの認識をもって診察する。(著者抄録)

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記述言語：日本語   出版者・発行元：(株)日本臨床社  

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記述言語：日本語   出版者・発行元：(株)東京医学社  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MEDICAL ASSOC NIPPON MEDICAL SCH  

C1q deficiency is a rare complement deficiency in the early part of the complement cascade. Patients with C1q deficiency have severe recurring life-threatening infections and systemic lupus erythematosus (SLE)-like symptoms. We report on a boy with recurrent life-threatening infections and SLE-like recurrent skin conditions before 2 years of age. Immunological studies revealed an undetectable level of C1q. No abnormality was observed in the urine, but renal biopsy showed segmental granulonephritis. However, the changes observed were atypical for SLE nephritis. This case of C1q deficiency was unusual because the SLE-like symptoms appeared earlier than that normally seen in complement deficiency. Therefore, this case provides insights into the development of autoimmune disease, particularly in the early phase of component deficiency, and in managing renal disease that may develop in the future. (J Nippon Med Sch 2011; 78: 322-328)

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MEDICAL ASSOC NIPPON MEDICAL SCH  

Hemoglobin H (HbH) disease is the severe nonfatal form of alpha-thalassemia syndrome. It is usually caused by molecular defects of 3 of 4 alpha-globin genes (-/-alpha) which cause alpha-globin expression to be decreased. HbH disease is rare in Japan. Here, we report on a 6-year-old girl with HbH disease who had profound hypochromatic and microcytic anemia. Analysis of the alpha-globin genes of the patient's family showed that the father, who was Japanese, had an abnormal gene with a 3.7-kb deletion (-alpha(3.7)/alpha alpha). and the mother, who was Filipino, had a deletion removing both a-globin genes of the Filipino type (-(FIL)/alpha alpha). Neither parent had anemia. The patient was found to have HbH disease with a heterozygous genetic abnormality (-(FIL)/-alpha(3.7)). Recently, the number of marriages of Japanese to natives of areas where thalassemia is epidemic has increased. Therefore, the incidence of HbH disease can be expected to increase in Japan. Long-term follow-up will be needed to evaluate the long-term complications and to improve the quality of life of patients with HbH disease. (J Nippon Med Sch 2011; 78: 101-104)

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記述言語：日本語   出版者・発行元：金原出版(株)  

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記述言語：日本語   出版者・発行元：(株)新興医学出版社  

●問診が重要である。●小児の貧血には年齢による特徴がある。●診断を進めていくには平均赤血球容積(mean corpuscular volume:MCV)で分類していくとわかりやすい。●鉄欠乏性貧血がもっとも頻度が高い。●貧血の有無にかかわらず鉄欠乏状態が発育・発達に影響を与えるといわれている。(著者抄録)

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記述言語：英語   出版者・発行元：TAYLOR & FRANCIS LTD  

DOI： 10.3109/10428194.2010.488707

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：INT PEDIATRIC RESEARCH FOUNDATION, INC  

We examined whether regenerated bone marrow (BM) with BM-derived stromal cells (BMSCs) on hydroxyapatite (HA) scaffolds can reconstitute the functional niche. Ly5.2 BMSCs on HA scaffolds were implanted s.c. onto the backs of Ly5.2 recipient mice. Lineage negative Ly5.1 BM cells expressing luciferase (luc) were i.v. administered into the recipient mice. Eight weeks after primary transplantation, secondary implantation was performed; the scaffolds removed from the first recipient mice were s.c. implanted into secondary recipient mice. Luc+ cells were detected in the scaffolds for 6 mo after secondary implantation. Injection of G-CSF resulted in wide distribution of bioluminescence from the original scaffolds to the whole body. Even after removing the scaffolds from the secondary recipient mice, luc+ cells were emitted by G-CSF stimulation, indicating that regenerated BM is capable of supporting hematopoietic stem cells (HSCs) and delivering HSCs to native BM in vivo. These data suggest that the functional niche is reconstituted at least partly and that regenerated BM on the scaffold may be used as a portable source of HSCs. (Pediatr Res 68: 35-40, 2010)

DOI： 10.1203/PDR.0b013e3181e1cfce

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Obstructive sleep apnea syndrome affects 1% to 2% of children. It is caused mainly by upper airway obstruction and manifests as snoring and sleep disturbance. Adenotonsillectomy can improve quality of life because airway obstruction occurs when both tonsils and adenoids are enlarged. We describe an 8-year-old girl with a recurrence of obstructive sleep apnea syndrome caused by hypertrophy of the tubal tonsils 4 years after adenotonsillectomy. The findings from this case highlight the importance of 1) identifying hypertrophy of the residual adenoid and compensatory hypertrophy of the tubal tonsils in patients with obstructive sleep apnea syndrome after adenotonsillectomy and 2) determining the optimal timing of adenotonsillectomy with respect to both the severity of obstructive sleep apnea and compensatory hypertrophy of other lymphoid tissue of Waldeyer's ring, as growth of such tissues is most active during the first several years of life.

DOI： 10.1272/jnms.77.265

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACADEMIC PRESS INC ELSEVIER SCIENCE  

Full-term cord blood (TCB) hematopoietic stem/progenitor cells (HSC/HPCs) are used for stem cell transplantation and are well characterized. However, the properties of preterm cord blood (PCB) HSC/HPCs remain unclear. In the present study, we compared HSC/HPCs from TCB and PCB with respect to their expression of surface markers, homing capacity and ability to repopulate HSCs in the NOD/Shi-scid mice bone marrow. The proportion of CD34+CD38- cells was significantly higher in PCB. On the other hand, the engraftment rate of TCB CD34+ cells into NOD/Shi-scid mice was significantly higher than PCB CD34+ cells. The expression of VLA4 was stronger among TCB CD34+ cells than PCB CD34+ cells. Moreover, there was a positive correlation between the proportion of CD34+CXCR4+ cells and gestational age. These data suggest that the homing ability of HSCs increases during gestation, so that TCB may be a better source of HSCs for transplantation than PCB. (C) 2009 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2009.08.139

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

Although the homing of hematopoietic stem cells (HSC) to the bone marrow (BM) is a crucial step in hematopoietic development and BM repopulation, the mechanisms underlying these processes have not been fully clarified. Recent studies suggest that interaction between the chemokine receptor CXCR4 and its ligand, stromal cell-derived factor 1 (SDF-1), plays a critical role in these processes. In addition, dextran sulfate increases plasma SDF-1 levels in mice and nonhuman primates. Thus, we examined the effects of preconditioning with SDF-1 and dextran sulfate on the homing efficiency of HSCs following BM transplantation in mice. We found that the preconditioning of donor mice with either SDF-1 or dextran sulfate enhanced the homing efficiency of infused HSCs in vivo. The greatest effects were obtained with dextran sulfate. Moreover, reverse transcriptase polymerase chain reaction analysis demonstrated that SDF-1 and dextran sulfate increased transcription of a variety of homing-related genes, including those for CXCR4, lymphocyte function associated antigen-1, matrix metalloproteinase-9, very late antigen-4/5, and macrophage inflammatory protein-1. We suggest that whereas SDF-1 directly acts to upregulate CXCR4 expression in HSCs, dextran sulfate acts via multiple pathways involved in the induction of various homing-related molecules, in addition to SDF-1. Thus, preconditioning donors with dextran sulfate offers a novel clinical approach for improving the homing and engraftment of HSCs in the BM.

DOI： 10.1272/jnms.76.198

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記述言語：日本語   出版者・発行元：金原出版(株)  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MARY ANN LIEBERT INC  

Inherited disorders of globin synthesis remain desirable targets for hematopoietic stem cell (HSC)-based therapies. Gene transfer using retroviral vectors offers an alternative to allogeneic HSC transplantation by the permanent integration of potentially therapeutic genes into primary autologous HSCs. Although proof of principle has been demonstrated in humans, this approach has been met by formidable obstacles, and large-animal models have become increasingly important for the preclinical development of gene addition strategies. Here we report lentiviral gene transfer of the human beta-globin gene under the control of the globin promoter and large fragments of the globin locus control region (LCR) in the nonhuman primate. Using an HIV-1, vesicular stomatitis virus glycoprotein G (VSV-G)-pseudotyped vector, modified to overcome a species-specific restriction to HIV-1, gene transfer to colony-forming units (CFU) derived from mobilized peripheral blood (PB) rhesus CD34(+) cells was 84.4 +/- 2.33%. Erythroid cells derived from transduced rhesus CD34(+) cells expressed human beta-globin at high levels as assessed by flow cytometry with a human beta-globin-specific antibody. Two rhesus macaques (RQ3586 and RQ3583) were transplanted with mobilized PB CD34(+) cells transduced with our modified HIV vector at a multiplicity of infection of 80. High gene transfer rates to CFUs were achieved in vitro (RQ3586, 87.5%; RQ3583, 83.3%), with efficient human beta-globin expression among erythroid progeny generated in vitro. Early posttransplantation, gene transfer rates of 5% or higher were detectable and confirmed by genomic Southern blotting, with equivalent-level human beta-globin expression detected by flow cytometry. Long-term gene marking levels among mononuclear cells and granulocytes assessed by quantitative polymerase chain reaction gradually decreased to about 0.001% at 2 years, likely due to additional HIV-1 restrictive elements in the rhesus macaque. No evidence of clonal hematopoiesis has occurred in our animals in up to 2 years. Current efforts are aimed at developing a lentiviral vector capable of efficiently transducing both human and rhesus HSCs to allow preclinical modeling of globin gene transfer.

DOI： 10.1089/hum.2008.186

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

We report a case of Diphyllobothrium nihonkaiense infection in a 2-year-old Japanese girl. When infection occurs in early childhood, it is necessary to offer supportive care in addition to standard treatment with oral praziquantel or with of a duodenal tube using a radiopaque contrast medium. We treated D. nihonkaiense infection in a 2-year-old girl successfully treated with oral praziquantel and a laxative.

DOI： 10.1272/jnms.75.225

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記述言語：日本語   出版者・発行元：日本医科大学医学会  

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2008&ichushi_jid=J04260&link_issn=&doc_id=20080623400005&doc_link_id=10.1272%2Fmanms.4.136&url=https%3A%2F%2Fdoi.org%2F10.1272%2Fmanms.4.136&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

記述言語：日本語   出版者・発行元：金原出版(株)  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BLACKWELL PUBLISHING  

DOI： 10.1111/j.1442-200X.2007.02470.x

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記述言語：英語   出版者・発行元：AMER SOC HEMATOLOGY  

DOI： 10.1182/blood.V110.11.1419.1419

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記述言語：日本語   出版者・発行元：(株)診断と治療社  

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掲載種別：研究論文（学術雑誌）  

The prognosis and clinical and biological characteristics of infant leukemia differ from those of leukemia in children 1 year or older. We reviewed the charts of patients younger than 1 year in whom leukemia was diagnosed from January 1981 through December 2003 at our institution. Fourteen infants had leukemia, 6 had acute lymphoblastic leukemia (ALL), and 8 had acute myeloid leukemia (AML). The age of patients at diagnosis ranged from 2 to 11 months. Five of 8 AML patients presented with cutaneous manifestations, such as erythema and nodules, at diagnosis. Central nervous system (CNS) involvement was seen in 1 AML patient at diagnosis. Hyperleukocytosis of more than 50 × 10 9/L was seen in 4 of 6 ALL patients and in 4 of 8 AML patients at diagnosis. All ALL patients showed a morphological diagnosis of L1 using the French-America-British classification system. For patients with AML, the morphological diagnoses were M0 for 1 patient, M2 for 1 patient, M4 for 2 patients (1 with eosinophilia), M5b for 2 patients, and M7 for 2 patients. One patient showing M7 morphology had Down syndrome. Surface markers were examined in 5 of 6 ALL patients and all AML patients. Five ALL patients showed a B-cell precursor immunophenotype. Two of 5 patients with ALL had CD10-positive leukemic cells and 3 of 5 patients with ALL had CD10-negative leukemic cells. All AML patients were positive for CD13 or CD33 or both. Three of 5 patients with ALL showed abnormal chromosomes related to 11q. Six of 7 patients with AML showed abnormal karyotypes. MLL gene rearrangements were seen in 3 (2 ALL, 1 AML) of 5 (2 ALL, 3 AML) patients. Serum immunoglobulin M levels were increased in 9 of 14 patients. Complete remission (CR) was achieved in all infants with ALL. Three patients relapsed and then died of the original disease. One of these 3 patients died after cord blood transplantation. Three ALL patients are alive without leukemia. CR was achieved in 6 of 8 AML patients. Four of 6 patients are alive without leukemia. Infant leukemia patients in our institution had some special features. CNS involvement at diagnosis was seen in only 1 patient and serum IgM levels were higher than those in children whose leukemia was diagnosed at 1 to 10 years of age.

DOI： 10.1272/jnms.72.355

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

We sought to determine whether hepatic side population (SP) cells derived from adult human liver possess the potential of a novel candidate hepatic stem cell. Human cadaveric donor liver was subjected to collagenase perfusion and hepatocytes were separated from nonparenchymal cells by differential centrifugation. SP cells were isolated from the nonparenchymal portion after Hoechst 33342 staining. Since CD45 is a panleukocyte antigen, CD45-negative SP cells were separated from the vast majority of CD45-positive SP cells (90%), and hepatic growth medium was used to culture both groups. Both CD45-negative and CD45-positive hepatic SP cells generated colonies in the hepatic growth medium in 2-3 weeks. The colonies yielded large cells morphologically consistent with human hepatocytes, demonstrating granule-rich cytoplasm, dense, often double nuclei, and intracellular lipofuscin pigment. The cultured cells from both sources were positive for markers of human hepatocytes: HepPar, cytokeratin 8 (CK8), and human albumin. Reverse transcriptase-polymerase chain reaction (RT-PCR) performed on both groups demonstrated positivity for additional liver markers including human albumin, CK18, alpha-1 anti-trypsin, and the human cytochrome P450 enzyme CYP2B6. Double immunostaining (CD45 and HepPar) and RT-PCR confirmed that the hepatocyte-like cells derived from the CD45-negative SP cells acquired HepPar positivity but had no detectable CD45 antigen expression. In contrast, the cultured cells derived from the CD45-positive SP cells also acquired HepPar positivity, but only a minimal fraction expressed the CD45 antigen. We conclude that hepatic SP cells derived from the nonparenchymal portion of human liver are a potential source of human hepatocytes irrespective of their CD45 status, and further animal studies will be required to assess their regenerative potential.

DOI： 10.1007/s10620-005-2933-x

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1272/jnms.72.252

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記述言語：日本語   出版者・発行元：千代田開発(株)  

12歳3ヵ月男児.患者はB前駆型急性リンパ性白血病と診断され,東京小児癌研究グループのL99-15HRプロトコール治療を開始した.第2コース治療終了後に好中球減少を認め,lenograstim投与を開始した.第2回目の投与から数時間後,左側肘関節,膝関節,臀部に有痛性紅斑の主訴を認めた.Lenograstimの過敏症を疑い,投与を中止したが症状に変化はなく,投与を再開した.この有痛性紅斑はlenograstim終了翌日になっても消失せず,以前に使用されていたcytarabinの影響も疑った.化学療法3コース目終了後,再び好中球減少し,lenograstimを投与すると,右大腿部近位部に有痛性結節を認めた.Cytarabinは使用していなかったので,顆粒球コロニー刺激因子製剤の副作用を疑い,filgrastimに変更した.Filgrastimでも大きな変化は認めず,nartograstimに変更した結果,皮膚過敏症はなく,数日後に好中球数が回復した.以後,好中球数減少時にはnartograstimを投与しているが,有害事象は認めていない

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC  

Hematopoietic cells can be highly enriched for repopulating ability based upon the efflux of the fluorescent Hoechst 33342 dye by sorting for SP ( side population) cells, a phenotype attributed to expression of ABCG2, a member of the ABC transporter superfamily. Intriguingly, murine studies suggest that forced ABCG2 expression prevents hematopoietic differentiation. We cloned the full-length rhesus ABCG2 and introduced it into a retroviral vector. ABCG2-transduced human peripheral blood progenitor cells (PBPCs) acquired the SP phenotype but showed significantly reduced growth compared with control. Two rhesus macaques received autologous PBPCs split for transduction with the ABCG2 or control vectors. Marking levels were similar between fractions with no discrepancy between bone marrow and peripheral blood marking. Analysis for the SP phenotype among bone marrow and mature blood populations confirmed ABCG2 expression at levels predicted by vector copy number long term, demonstrating no block to differentiation in the large animal. In vitro studies showed selective protection against mitoxantrone among ABCG2-transduced rhesus PBPCs. Our results confirm the existence of rhesus ABCG2, establish its importance in conferring the SP phenotype, suggest no detrimental effect of its overexpression upon differentiation in vivo, and imply a potential role for its overexpression as an in vivo selection strategy for gene therapy applications.

DOI： 10.1074/jbc.M409796200

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：CARDEN JENNINGS PUBL CO LTD  

Studies have indicated that bone marrow contains both hematopoietic stem cells and mesenchymal stem cells that can differentiate into a variety of mesenchymal tissues, such as bone, cartilage, muscle, and adipose tissue. Therefore, bone marrow cells are thought to be very useful for cell and gene therapy for various diseases. However, the multipotentiality of these cells remains unclear. To address this issue, we established a chimeric model mouse stably reconstituted with green fluorescent protein (GFP)-marked bone marrow cells. We injected bone marrow cells from GFP-transgenic C57BL/6 mice into the tail veins of recipient wild-type C57BL/6 mice that had been irradiated with a lethal dose of 10 Gy from a cesium source. Microscopic examination and fluorescence-assisted cell sorter (FACS) analysis showed that bone marrow cells, including mesenchymal cells, were almost completely reconstituted with GFP1 cells 5 weeks after transplantation. FACS analysis with lineage-specific antibodies confirmed that the GFP1 cells could differentiate into all types of blood cells. To confirm the usefulness of this mouse model, we studied the role of circulating bone marrow-derived cells in healing of damaged intestine. We performed amputation and anastomosis of the jejunum 10 cm from the pyloric region of the stomach. On the third day after operation, a large number of GFP(+) cells were infiltrated in the area of anastomosis, and these cells were positive for CD45 and F4/80 antigens. In 7 days, several cells became negative for CD45 and F4/80 and positive for alpha smooth muscle actin antigen, which is specific for smooth muscle. This finding suggested that bone marrow-derived cells had differentiated into smooth muscle. Because reconstituted bone marrow cells, as opposed to injected bone marrow cells, behave naturally, this model is ideal for studying the multipotentiality of bone marrow cells in vivo. (C)2003 The Japanese Society of Hematology.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-LISS  

DOI： 10.1002/mpo.10228

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DOI： 10.1385/1-59259-345-3:387

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BLACKWELL PUBLISHING LTD  

In the present study, we examined the expression of Flk2/Flt3, a tyrosine kinase receptor, on human cord blood CD34(+) haematopoietic progenitor/stem cells. In flow cytometric analysis, Flk2/Flt3 was expressed on 80% of CD34(+) cells and their immature subpopulations, CD34(+) CD33(-) and CD34(+) CD38(-) cells. Methycellulose clonal culture of sorted CD34(+) Flk2/ Flt3(+) and CD34(+) Flk2/Flt3(-) cells showed that most of myelocytic progenitors expressed Flk2/Flt3, but erythroid and haematopoietic multipotential progenitors were shared by both fractions. When 1 x 10(4) lineage marker-negative (Lin(-))CD34(+)Flk2/Flt3(-) cells were transplanted into non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice, none of the recipients possessed human CD45(+) cells in bone marrow 11-12 weeks after the transplantation. In contrast, all recipients transplanted with 1 x 10(4) Lin(-) CD34(+) Flk2/Flt3(+) cells showed successful engraftment. Furthermore, clonal cells expanded from single Lin(-) CD34(+) CD38(-) Flk2/Flt3(+) cells in the culture with Flk2/Flt3 ligand, stem cell factor, thrombopoietin, and a complex of interleukin 6/soluble interleukin 6 receptor were individually transplanted into NOD/SCID mice. At 20 to 21 weeks after the transplantation, three out of 10 clones harvested at d 7 of culture, and three out of six clones at d 14 could reconstitute human haematopoiesis in recipient marrow. These results demonstrated that Flk2/Flt3 was expressed on a wide variety of human haematopoietic cells including long-term-repopulating haematopoietic stem cells.

DOI： 10.1046/j.1365-2141.2002.03820.x

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：TAYLOR & FRANCIS AS  

We diagnosed influenza infection in 2 children receiving maintenance treatment for acute lymphoblastic leukemia. Both patients received zanamivir within 1 d of the onset of fever and their symptoms of influenza were rapidly alleviated. We conclude that inhaled zanamivir seems to be an effective treatment for influenza infection in immunocompromised patients.

DOI： 10.1080/00365540210147714

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記述言語：日本語   出版者・発行元：日本小児血液学会  

11歳男児.左鼠径部に径6cmの腫瘤を認めたため入院した.各種画像検査により腹腔内リンパ腫大も認められたが,横隔膜より上部のリンパ節の腫大は認められず,発熱,盗汗,体重減少等の症状も認められなかった.リンパ節生検を行い,結節性硬化の組織型を呈するホジキン病(ステージIIA)と診断した.COPP/ABVD療法による化学療法を行い,治療開始後約7ヵ月で治療を終了した.現在,発症から1年6ヵ月が経過しているが,再発を認めていない

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2001&ichushi_jid=J02258&link_issn=&doc_id=20011107080009&doc_link_id=10.11412%2Fjjph1987.15.405&url=https%3A%2F%2Fdoi.org%2F10.11412%2Fjjph1987.15.405&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：CARDEN JENNINGS PUBL CO LTD  

The characteristics of hematopoietic progenitor and stem cell (HPC/HSC) populations in mammals vary according to their ontogenic stage. In humans, HPC/HSCs from umbilical cord blood (CB) are increasingly used as an alternative to HPC/HSCs from adult bone marrow (BM) for the treatment of various hematologic disorders. How the hematopoietic activity of progenitor and stem cells in CB differs from that in adult BM remains unclear, however. We compared CD34(+) cells, a hematopoietic cell population, in CB with those in adult BM using phenotypic subpopulations analyzed by Row cytometry, the colony- forming activity in methylcellulose clonal cultures, and the repopulating ability of these: cells in NOD/Shi-scid (NOD/SCID) mice. Although the proportion of CD34(+) cells was higher in adult BM than in CB mononuclear cells, the more immature subpopulations, CD34(+)CD33(-) and CD34(+)CD38(-) cells, were present in higher proportions in CD34(+) CB cells. Clonal culture assay showed that more multipotential progenitors were present in CD34(-) CB cells. When transplanted into NOD/SCID mice, CD34(+) adult BM cells could not reconstitute human hematopoiesis in recipient BMI but CD34- CB cells achieved a high level of engraftment, indicating that CD34(+) CB cells possess a greater repopulating ability. These results demonstrated that human hematopoiesis changes with development from fetus to adult. Furthermore. CD34(+) CB cells contained a greater number of primitive hematopoietic cells, including HSCs, than did adult BM, suggesting the usefulness of CD34(+) CB cells not only as a graft for therapeutic HSC transplantation but also as a target cell population for ex vivo expansion of transplantable HSCs and for gene transfer in gene therapy. Int J Hematol. 2001;73:457-462. (C) 2001 The Japanese Society of Hematology.

DOI： 10.1007/BF02994007

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

We describe here the case of an 8-year-old girl with Fanconi anemia (FA) whose hematopoiesis was successfully restored by unrelated umbilical cord blood (UCB) transplantation. The patient became resistant to androgen therapy, and developed intracranial hemorrhage and dyserythropoiesis. Her hematopoietic recovery after the transplantation was excellent and a complete chimerism has been durably maintained. UCB should be considered as a stem cell source for transplantation when a patient with FA does not have an HLA-identical unaffected sibling donor.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER SOC HEMATOLOGY  

The CD34 antigen serves as an important marker for primitive hematopoietic cells in therapeutic transplantation of hematopoietic stem cells (HSC) and gene therapy, but it has remained an open question as to whether or not most HSC express CD34, Using a competitive long-term reconstitution assay, the results of this study confirm developmental changes in CD34 expression on murine HSC, In fetuses and neonates, CD34 was expressed on Lin(-)c-Kit(+) long-term repopulating HSC of bone marrow (BM), liver, and spleen. However, CD34 expression on HSC decreased with aging, and in mice older than 10 weeks, HSC were most enriched in the Lin(-)c-Kit(+)CD34(-) marrow cell fraction. A second transplantation was performed from primary recipients who were transplanted with neonatal Lin(-)c-Kit(+) CD34(high) HSC marrow. Although donor-type HSC resided in CD34-expressing cell fraction in BM cells of the first recipients 4 weeks after the first transplantation, the stem cell activity had shifted to Lin(-)c-Kit(+)CD34(-) cells after 16 weeks, indicating that adult Lin-c-Kit(+)CD34- HSC are the progeny of neonatal CD34-expresssing HSC, Assays for colony-forming cells showed that hematopoietic progenitor cells, unlike HSC, continue to express CD34 throughout murine development. The present findings are important because the clinical application of HSC can be extended, in particular as related to CD34-enriched HSC and umbilical cord blood HSC, (C) 2001 by The American Society of Hematology.

DOI： 10.1182/blood.V97.2.419

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記述言語：英語   出版者・発行元：The Medical Association of Nippon Medical School  

An 11-year-old girl with Kostmann syndrome developed refractory pneumonia. Culture of oral discharge, throat-swab specimens, and blood could not identity the causative organism, and systemic antimicrobial therapy failed to achieve improvement. We then performed diagnostic bronchoalveolar lavage (BAL) and culture of BAL fluid (BALF) yielded Pseudomonas aeruginosa. Therapeutic BAL using gentamicin produced a striking improvement of her pneumonia.<br> Conclusion: In immunocompromised children with pneumonia, BAL helps to identify the causative organism. If the patient is unresponsive to systemic antimicrobial therapy, BAL using antimicrobial agents is also worth trying.<br>

DOI： 10.1272/jnms.68.340

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記述言語：日本語   出版者・発行元：日本医科大学医学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

The scid mutation was backcrossed on to the NOD/Shi mouse background, resulting in the development of NOD/Shi-scid mice, which showed lack of mature lymphocytes, macrophage dysfunction and absence of circulating complement, but were not as impaired in natural killer (NK) cell activity as NOD/LtSz-scid mice. We then examined the effect of recipient NK cell depletion by anti-asialo GM1 antiserum on the repopulation of human cord blood (CB) hematopoietic stem cells (HSC) in NOD/Shi-scid mice to clarify the role of recipient NK cells in human HSC engraftment. The anti-asialo GM1 antiserum treatment significantly enhanced the engraftment of CB CD34(+) cells, but did not affect the differentiation of the engrafted HSC into each hematopoietic lineage. The NK cell depletion was effective at early stages of the engraftment, but not 3 weeks after the transplantation. The anti-asialo GM1 antiserum treatment did not improve the engraftment by human HSC in scid mice which lack mature lymphocytes, but show neither macrophage dysfunction nor a reduction in circulating complement, indicating that macrophages and/or complement also have roles in HSC graft rejection. The present study indicates that the preconditioning targeting of recipient NK cells in addition to T cell suppression and myeloablation might prevent HSC graft failure, and that NOD/Shi-scid mice treated with anti-asialo GM1 antiserum could provide a useful tool for evaluating the repopulating ability of transplantable human HSC.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：NATURE PUBLISHING GROUP  

We report a 13-year-old boy who developed dyspnea at rest 1 year after the occurrence of cGVHD following an allogeneic bone marrow transplant (BMT), Pulmonary function data, imaging studies, lung biopsy, and bronchoalveolar lavage were consistent with the diagnosis of bronchiolitis obliterans organizing pneumonia (BOOP), Although reports suggest that oral methylprednisolone or methylprednisolone pulse therapies improve BOOP after BMT, we treated our patient with a combination of oral prednisolone (1 mg/kg) and low dose erythromycin (10 mg/kg) to avoid the side-effects of high-dose steroids. With this therapy, our patient showed clinical and radiological improvements within 1 week, The steroids were tapered off 12 months later and erythromycin was given for 14 months, We conclude that therapy consisting of a combination of oral prednisolone and low-dose erythromycin for BOOP after BMT may minimize the dose and duration of steroid use.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：CARDEN JENNINGS PUBL CO LTD  

A 10-year-old girl presented with massive pericardial/pleural effusion with anasarca 216 days after an allogeneic bone marrow transplantation from her HLA-matched sibling for relapsed acute lymphoblastic leukemia. She did not show any other symptoms of chronic graft-versus-host disease (GVHD). The antinucleolar antibody was elevated in the blood and the pleural fluid. The lymphocytes in the fluid were mostly CD8(+)/HLA-DR+, and a majority of CD8(+) cells in the blood expressed CD57. These data suggested that she had chronic GVHD. Immunosuppressive therapy including prednisolone, cyclosporin A, high-dose methylprednisolone, tacrolimus (FK506), and methotrexate had no effect, and the patient died of Aspergillus pneumonia 183 days after the presentation of the disease. Although it has not been described before, isolated serositis with edema should be recognized as a clinical feature of chronic GVHD. Int J Hematol. 2000;71:394-397. (C) 2000 The Japanese Society of Hematology.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：AMER SOC CLINICAL INVESTIGATION INC  

Here, we demonstrate a significant ex vivo expansion of human hematopoietic stem cells capable of repopulating in NOD/SCID mice. Using a combination of stem cell factor (SCF), Flk2/Flt3 ligand (FL), thrombopoietin (TPO), and a complex of IL-6 and soluble IL-6 receptor (IL-6/sIL-6R), we cultured cord blood CD34(+) cells for 7 days and transplanted these cells into NOD/SCID mice. Bone marrow engraftment was judged successful when recipient animals contained measurable numbers of human CD45(+) cells 10-12 weeks after transplantation When cells were cultured with SCF+FL+TPO+IL-6/sIL-6R, 13 of 16 recipients were successfully engrafted, and CD45(+) cells represented 11.5% of bone marrow cells in engrafted recipients. Cells cultured with a subset of these factors were less efficiently engrafted, both as measured by frequency of successful transplantations and prevalence of CD45(+) cells. In animals receiving cells cultured with all 4 factors, human CD45(+) cells represented various lineages, including a large number of CD34(+) cells. The proportion of CD45(+) cells in recipient marrow was 10 times higher in animals receiving these cultured cells than in those receiving comparable numbers of fresh CD34(+) cells, and the expansion rate was estimated at 4.2-fold by a limiting dilution method. Addition of IL-3 to the cytokine combination abrogated the repopulating ability of the expanded cells. The present study may provide a novel culture method for the expansion of human transplantable hematopoietic stem cells suitable for clinical applications.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ALPHAMED PRESS  

Although umbilical cord blood (CB) is increasingly being used as an alternative to bone marrow (BM) as a source of transplantable hematopoietic stem cells (HSC), information on the hematopoietic repopulating ability of CB HSC is still limited. We recently established a xeno; transplantation system in NOD/Shi-acid mice to evaluate human stem cell activity. In the present study, we transplanted 5 to 10 x 10(4) CB CD34(+) cells into six NOD/Shi-scid mice treated with anti-asialo GM1 antiserum to investigate the hematopoietic repopulating ability of CB, The BM of all recipients contained human CD45(+) cells 10 to 12 weeks after the transplantation (43.8 +/- 17.%). Clonal culture of the recipient BM cells revealed the formation of various types of human hematopoietic colonies, including myelocytic, erythroid, megakaryocytic, and multilineage colonies, indicating that CB HSC can differentiate into hematopoietic progenitors of various lineages. However, the extent of the differentiation and maturation differed with each lineage. CD13(+)D14(+)CD33(+) myelocytic cells were mainly repopulated in BM and peripheral blood (PB), While CD41(+) megakaryocytic cells and platelets were present, few glycophorin A(+)CD71(+) or hemoglobin ex-containing erythroid cells were detected. CD19(+) B cells were the most abundantly repopulated in NOD/Shi-scid mice, but their maturational stage differed among the hematopoietic organs. Most of the BM CD19+ cells were immature B cells expressing CD10 but not surface immunoglobulin (Ig) M, whereas more mature CD19(+)CD10(-) surface IgM(+) B cells were predominantly present in spleen and PB, CD3(+) T cells were not detected even in the recipient thymus. The transplantation to the NOD/Shi-scid mouse may provide a useful tool for evaluating the repopulating ability of transplantable human HSC.

DOI： 10.1634/stemcells.18-3-204

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：KLUWER ACADEMIC PUBL  

To better characterize skeletal complications in Japanese patients with type I Gaucher disease (GD), we performed genotyping and clinical and radiological analysis of 35 patients, the vast majority of this population, Skeletal complications tend to be very common, severe and rapidly progressive in Japanese patients with type 1 GD. Twenty (57%) of these patients manifested end points of severe bone disease including avascular necrosis, pathological fracture and/or bone crisis. Mean time from presentation/diagnosis of GD until presentation of this involvement was 3 years 6 months +/- 4 years I month. Prevalence of severe bone disease is significantly higher in splenectomized than in non-splenectomized patients-81% (17/21) versus 21% (3/14) (p = 0.0007, Fisher's exact test). Four (29%) of 14 patients receiving enzyme replacement therapy (ERT) or bone marrow transplantation (BMT) manifested severe bone involvement for the first time during or after treatment. All cases occurred in children in whom ERT doses had been lowered after only brief administration of higher starting doses (n = 3) or partial donor marrow engraftment resulted in low glucocerebrosidase (GCR) activity (n = 1). These observations suggest that splenectomy may correlate with accelerated skeletal deterioration with severe skeletal disease, at least in patients with severe phenotypic expression. They also suggest that it is important that sufficient GCR is available in paediatric patients with severe phenotypic expression. Hence ERT dosages should be based on disease severity and on age, with sustained administration of full doses in patients at greater risk of important skeletal complications.

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記述言語：日本語   出版者・発行元：日本小児血液学会  

2症例(14歳男児,9歳男児)において,多分割高線量の全身放射線照射(13.5Gy,9分割)を含む前処置を用いた同種骨髄移植を施行した.移植病期は症例1が血液学的再発期,症例2がPCRレベルでの再発期であり,ドナーは症例1が非血縁者,症例2がHLA部分不一致の母親であった.症例1ではday 14,症例2ではday 12に生着を確認し,骨髄のBCR/ABLのメッセージは症例1ではday 89,症例2ではday 19に消失し以後,再出現することはなかった.症例1ではIV度,症例2ではIII度のGVHDがみられ,又,2例ともアデノウイルス11型による出血性膀胱炎を発症した.症例1は間質性肺炎の為day442で死亡したが,症例2はday 231現在無病生存中である

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=1998&ichushi_jid=J02258&link_issn=&doc_id=19990114040011&doc_link_id=10.11412%2Fjjph1987.12.452&url=https%3A%2F%2Fdoi.org%2F10.11412%2Fjjph1987.12.452&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：W B SAUNDERS CO  

We report here on a novel stromal cell line, AGM-S3, derived from the aorta-gonad-mesonephros (AGM) region of a 10.5 days postcoitum (dpc) mouse embryo. The AGM-SB cells promoted production of hematopoietic progenitors and day-12 spleen colony-forming cells from Lin(-)c-Kit(+)Sca-1(+) murine primitive hematopoietic cells. They also supported for 6 weeks generation of human multipotential progenitors from cord blood CD34(+)CD38(-) primitive hematopoietic cells. Human long-term repopulating hematopoietic stem cells (LTR-HSC) with the potential to reconstitute hematopoiesis in NOD/SCID mice were maintained on AGM-SB cells for at least 4 weeks. Flow cytometric analysis showed that CD13, vascular cellular adhesion molecule-1, and Sca-1 were expressed on AGM-S3 cells. Because stem cell factor, interleukin-6 (IL-6), and oncostatin M, but not IL-3, IL-11, leukemia-inhibitory factor, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, thrombopoietin, and Flk2 ligand were detected in reverse transcription-polymerase chain reaction analysis of AGM-SB cells, the cells seem to express species-cross reactive molecule(s) other than the cytokines examined and which act on primitive hematopoietic progenitor/stem cells. This cell line is expected to elucidate molecular mechanisms regulating early hematopoiesis and pave the way for developing strategies for expansion of human transplantable HSC. (C) 1998 by The American Society of Hematology.

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

アドリアマイシン(ADR),エトポシド(VP-16),ビンクリスチン(VCR),デキサメタゾン(DEX)がB細胞株及び白血病細胞での細胞内酸化状態に及ぼす影響を,2'-7'-dichlorofluorescin diacetate(DCFH-DA)を用いたフローサイトメトリー法により検討した.B細胞株においてADR,VP-16は細胞内酸化状態を亢進させたが,VCR, DEXは亢進させなかった.ADRによる細胞内酸化状態の亢進を初発治療前の白血病細胞では認めたが,P-糖蛋白陽性の再発白血病細胞では認めなかった.今回の研究で,DCFH-DAを用いた本法は抗癌剤による細胞内酸化状態の変化を簡便に測定できることを示した

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その他リンク： https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=1996&ichushi_jid=J02258&link_issn=&doc_id=19961031510003&doc_link_id=10.11412%2Fjjph1987.10.351&url=https%3A%2F%2Fdoi.org%2F10.11412%2Fjjph1987.10.351&type=J-STAGE&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00007_3.gif

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BLACKWELL SCIENCE  

In recent years, enzyme replacement therapy has been shown to be useful for the treatment of Gaucher disease. A 10 year old Japanese boy with Gaucher disease underwent splenectomy at the age of 5 years and received enzyme replacement therapy from the age of 6 years. He had avascular necrosis of the bilateral femoral heads, which was not seen at the beginning of the therapy, without deterioration of hematological variables during maintenance therapy. The enzyme dosage was increased from 20 to 120 IU/kg per month resulting in an improvement of the clinical symptoms and bone lesion. In enzyme replacement therapy, dose increase is considered to be essential for improvement in bone disease; however, it is important to watch for the development of bone lesion.

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In recent years, enzyme replacement therapy has been shown to be useful for the treatment of Gaucher disease. A 10 year old Japanese boy with Gaucher disease underwent splenectomy at the age of 5 years and received enzyme replacement therapy from the age of 6 years. He had avascular necrosis of the bilateral femoral heads, which was not seen at the beginning of the therapy, without deterioration of hematological variables during maintenance therapy. The enzyme dosage was increased from 20 to 120 IU/kg per month resulting in an improvement of the clinical symptoms and bone lesion. In enzyme replacement therapy, dose increase is considered to be essential for improvement in bone disease; however, it is important to watch for the development of bone lesion. © 1996 Japan Pediatric Society.

DOI： 10.1111/j.1442-200X.1996.tb03482.x

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記述言語：日本語   出版者・発行元：特定非営利活動法人 日本小児外科学会  

DOI： 10.11164/jjsps.32.6_977_4

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記述言語：日本語   出版者・発行元：(株)日本小児医事出版社  

症例1は1歳10ヵ月の男児,症例2は1ヵ月の男児.2例ともT細胞の成熟障害に顔貌の異常,副甲状腺機能低下症がみられた.症例1では心奇形は認めず,症例2は総動脈幹症を認めた.両症例ともFISH法による染色体分析にて,22番染色体q11の欠失を認めCATCH22症候群と診断した

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Medical Association of Nippon Medical School  

Gaucher disease is the most prevalent lysosomal storage disease. Although the efficacy of the macrophage-targeted human placental glucocerebrosidase is well known, it is still difficult to develop definitive guidelines regarding the appropriate therapy schedule. We describe an 8-year-old Japanese boy with Gaucher disease who had avascular necrosis of the right femoral head without deterioration of hematological variables during low-dose enzyme replacement therapy (12-13 IU/kg). This case demonstrates that continuous normal hematological findings may not preclude progression of other aspects of Gaucher disease in some patients during enzyme replacement therapy.

DOI： 10.1272/jnms1923.61.633

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記述言語：日本語   出版者・発行元：特定非営利活動法人 日本小児外科学会  

DOI： 10.11164/jjsps.30.5_1018_1

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記述言語：日本語   出版者・発行元：(株)診断と治療社  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

4歳8ヵ月男児.感冒症状に引続き強度の溶血性貧血と腎不全所見を呈して発症した.直接Coombs試験陽性,梅毒血清反応陰性,Donath-Landsteiner(D-L)抗体陽性であり,急性腎不全を合併した非梅毒性発作性寒冷血色素尿症と診断した.治療としては血液透析,血漿交換,輸血等を要したが,第7病日に溶血発作はみられなくなり,第9病日に血液透析より離脱し,第13病日にD-L抗体も陰性化した.組織学的検索の結果,近位尿細管にhemoglobin, hemosiderinの沈着が認められた

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記述言語：日本語   出版者・発行元：(一社)大阪小児科医会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(株)総合医学社  

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その他リンク： https://search.jamas.or.jp/default/link?pub_year=2022&ichushi_jid=J00643&link_issn=&doc_id=20220428280026&doc_link_id=%2Fag1snrsd%2F2022%2F007502%2F026%2F0331-0335%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fag1snrsd%2F2022%2F007502%2F026%2F0331-0335%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

記述言語：日本語   出版者・発行元：日本医科大学医学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本癌治療学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：英語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：日本医科大学医学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：英語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：英語   出版者・発行元：(一社)日本小児血液・がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児リウマチ学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児放射線学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児血液・がん学会・(NPO)日本小児がん看護学会・(公財)がんの子供を守る会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児血液・がん学会・(NPO)日本小児がん看護学会・(公財)がんの子供を守る会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児血液・がん学会・(NPO)日本小児がん看護学会・(公財)がんの子供を守る会  

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記述言語：日本語   出版者・発行元：(公社)日本小児保健協会  

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記述言語：日本語   出版者・発行元：(一社)日本血栓止血学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：日本医科大学医学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児血液・がん学会・(NPO)日本小児がん看護学会・(公財)がんの子供を守る会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児血液・がん学会・(NPO)日本小児がん看護学会・(公財)がんの子供を守る会  

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記述言語：日本語   出版者・発行元：日本小児呼吸器学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(公社)日本新生児成育医学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：日本小児皮膚科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本周産期・新生児医学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：がんの子供を守る会  

CiNii Books

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=262281

記述言語：日本語   出版者・発行元：がんの子供を守る会  

CiNii Books

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=261876

記述言語：日本語   出版者・発行元：がんの子供を守る会  

CiNii Books

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その他リンク： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=262002

記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児感染症学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：日本医科大学医学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：日本先天代謝異常学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：日本小児呼吸器学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：(一社)日本周産期・新生児医学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER SOC HEMATOLOGY  

Web of Science

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：AMER SOC HEMATOLOGY  

Web of Science

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会  

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記述言語：日本語   出版者・発行元：(公社)日本小児科学会  

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記述言語：英語  

CiNii Books

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：日本産婦人科・新生児血液学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会  

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記述言語：日本語   出版者・発行元：(一社)日本小児放射線学会  

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記述言語：日本語   出版者・発行元：(NPO)日本小児がん学会  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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記述言語：日本語   出版者・発行元：(一社)日本血液学会-東京事務局  

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記述言語：日本語   出版者・発行元：日本小児血液学会  

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