記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER JAPAN KK  

Papillary thyroid carcinoma (PTC) is the most frequent thyroid carcinoma. PTC cell lines have been of considerable value in studying aspects of thyroid cancer, such as gene expression, cell proliferation, and differentiation. Here we report three novel PTC lines established from three patients with different backgrounds. Case 1 was a 38-year-old woman with PTC in the right thyroid lobe, with no metastasis. The cell line was established from the resection sample and named D-PTC. The cell line consisted of epithelial cells with few lysosomes and showed a pavement structure and follicular formation at confluency. There was a little pilling up. The secretion of free thyroxin (fT4) and thyroglobulin (Tg) was increased by TSH, or GH and IGF-I treatment. Case 2 was a 22-year-old woman with PTC initially in the right thyroid lobe, but 4 years after the right lobe resection, PTC metastasis was observed in left lobe. The cell line was established from a sample of the second resection and named UD-PTC. This cell line consisted of small epithelial cells with evident lysosomes and exhibited floating cell clusters. The secretion of fT4 and Tg was slightly increased by TSH, or GH and IGF-I treatment. Case 3 was an 85-year-old man with PTC and with acromegaly. Metastasis was observed at cervical lymph nodes. The cell line was derived from the metastasis region and named A-PTC. This cell line consisted of small epithelial cells and many lysosomes. The cells frequently showed pilling up. The secretion of fT4 and Tg was significantly increased by GH and IGF-I treatment. We have established three PTC cell lines with substantial variation in their phenotype. The cell lines may be useful for thyroid cancer research.

DOI： 10.1007/s13577-014-0088-9

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ENDOCRINE SOC  

GH deficiency is known to be clinically associated with a high incidence of nonalcoholic fatty liver disease, and this can be reversed by GH administration. Here we investigated the mechanistic basis for this phenomenon using engineered male mice lacking different signaling elements of the GH receptor, hepatic stat5a/b(-/-) mice and a mouse hepatoma line. We found deficient GH-dependent signal transducer and activator of transcription (STAT)-5 signaling correlates with steatosis, and through microarray analysis, quantitative PCR, and chromatin immunoprecipitation, identified putative targets of STAT5 signaling responsible for the steatosis seen on a normal diet. These targets were verified with liver-specific stat5a/b deletion in vivo, and in vitro we show that dominant-negative (DN) STAT5 increases lipid uptake in a mouse hepatoma line. Because loss of STAT5 signaling results in elevated STAT1 and STAT3 activity and intracellular lipid accumulation, we have used DN-STAT5a/b, DN-STAT1, constitutively active (CA)-STAT3, or addition of oleate/palmitate in the hepatoma line to assign which of these apply to individual targets in STAT5 signaling deficiency. These findings and published mouse models of steatosis enable us to propose elevated cd36, ppar gamma, and pgc1 alpha/beta expression as primary instigators of the steatosis along with elevated fatty acid synthase, lipoprotein lipase, and very low-density lipoprotein receptor expression. Decreased fgf21 and insig2 expression may also contribute. In conclusion, despite normal plasma free fatty acids and minimal obesity, absent GH activation leads to steatosis because activated STAT5 prevents hepatic steatosis. These results raise the possibility of low-dose GH treatment for nonalcoholic fatty liver disease. (Endocrinology 152: 181-192, 2011)

DOI： 10.1210/en.2010-0537

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ENDOCRINE SOC  

GH is generally believed to signal exclusively through Janus tyrosine kinases (JAK), particularly JAK2, leading to activation of signal transducers and activators of transcription (STAT), ERK and phosphatidylinositol 3-kinase pathways, resulting in transcriptional regulation of target genes. Here we report the creation of targeted knock-in mice wherein the Box1 motif required for JAK2 activation by the GH receptor (GHR) has been disabled by four Pro/Ala mutations. These mice are unable to activate hepatic JAK2, STAT3, STAT5, or Akt in response to GH injection but can activate Src and ERK1/2. Their phenotype is identical to that of the GHR(-/-) mouse, emphasizing the key role of JAK2 in postnatal growth and the minimization of obesity in older males. In particular, they show dysregulation of the IGF-I/IGF-binding protein axis at transcript and protein levels and decreased bone length. Because no gross phenotypic differences were evident between GHR(-/-) and Box1 mutants, we undertook transcript profiling in liver from 4-month-old males. We compared their transcript profiles with our 391-GHR truncated mice, which activate JAK2, ERK1/2, and STAT3 in response to GH but not STAT5a/b. This has allowed us for the first time to identify in vivo Src/ERK-regulated transcripts, JAK2-regulated transcripts, and those regulated by the distal part of the GHR, particularly by STAT5. (Molecular Endocrinology 24: 204-217, 2010)

DOI： 10.1210/me.2009-0233

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPAN SOC INTERNAL MEDICINE  

Acromegaly is characterized by chronic hypersecretion of growth hormone (GH) and is associated with increased mortality rate because of the potential complications such as cardiovascular disease, respiratory disease, or malignancy, which are probably caused by the long-term exposure of tissues to excess GH, for at least 10 years, before diagnosis and treatment.
A 22-year-old man with a 2-month history of fatigue was admitted to our hospital because of chest discomfort, dyspnea, and pitting edema of the lower limbs experienced over a 1-month period. On admission, his height and body weight were 186 cm and 138.5 kg, respectively, with a BMI of 39.8 kg/m(2). He showed acromegalic features and elevated serum GH and IGF-1 levels, which were 11.5 ng/mL and 960 ng/mL, respectively. There was no GH suppression in the 75-g oral glucose tolerance test. Pituitary magnetic resonance imaging (MRI) revealed microadenoma. Chest X-ray revealed cardiomegaly, and echocardiogram showed dilated left ventricular (LV) cavity and diffuse hypokinesis with extremely decreased ejection fraction (EF). He was diagnosed as having acromegaly with congestive heart failure from diastolic cardiomyopathy.
After the successful transsphenoidal resection of the pituitary adenoma, the level of GH was normalized. However, the cardiac dysfunction did not show any improvement even after the administration of beta-blockers, angiotensin-converting enzyme inhibitor (ACE-I), or diuretics. The patient was re-hospitalized, and he died of cardiac failure at the age of 25 years.
Patients with acromegaly have been reported to have about 30% higher mortality rate, and cardiovascular disease accounts for 60% of the deaths. We report a case of a patient with juvenile acromegaly who was diagnosed with severe cardiac failure at the time of diagnosis and failed to recover cardiac function even after the successful resection of the pituitary adenoma. Immediate diagnosis and treatment are required for better control of acromegalic cardiomyopathy.

DOI： 10.2169/internalmedicine.49.3972

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

Currently there is no good hepatocyte model for studying growth hormone (GH) function that reflects its normal physiological roles. Here we report the establishment of a functional hepatocyte cell line, SDRL-1, from the liver of young male spontaneous dwarf rats (SDR), with isolated GH deficiency. This line has been maintained in Dulbecco's Modified Eagle Medium (DMEM)/F12 medium supplemented with 10% fetal bovine serum (FBS) with retention of a near diploid karyotype for extended periods of time. When grown as a monolayer sheet, it displayed a pavement-like appearance and contact inhibition. These cells have a poorly developed rough endoplasmic reticulum (r-ER), few mitochondria and glycogen granules, and produce a small amount of albumin and alpha-fetoprotein, that is enhanced when grown on a collagen gel sponge. Human recombinant GH stimulated JAK2 and STAT5b tyrosine phosphorylation and IGF-I production in a concentration-dependent manner. When the cells were cultured with GH-supplemented medium, the number of mitochondria and glycogen granules increased together with the r-ER and Golgi apparatus. A number of microvilli were observed on the surface of the cultured cells, further suggesting that this cell line is composed of normally functioning hepatocytes. In summary, we established a novel hepatocyte cell line (SDRL-1), that appears to display normal function, which we propose can serve as a good in vitro model for studying GH-target organ interactions.

DOI： 10.1111/j.1749-0774.2010.00097.x

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER TOKYO  

There are few case reports describing small cell lung carcinoma (SCLC), which secrete parathyroid hormone (PTH)-related protein (PTHrP) and result in hypercalcemia. We have established a novel cell line, derived from a 37-year-old woman with SCLC, which produced PTH, PTH-rP, and a part of proopiomelanocortin (POMC), and led to hypercalcemia. The cell line, named SS-1, was grown as floating cell clusters in DMEM/F12 medium supplemented with 10% fetal bovine serum and had a population doubling time of 72 h. The modal chromosome number was 47 (88%); marker chromosomes were not observed. The SS-1 cell line secreted not only PTHrP but also PTH, and both were decreased by CaCl(2) administration. Decreasing the concentration of Ca++ in the growth medium stimulated the secretion of both PTHrP and PTH. The cell line had calcium sensing receptor (Cas-R). Since PTHrP and PTH secretion from the SS-1 cells was related to Ca++ concentration in the growth medium, the cell line might be useful for the study of PTH-rP and PTH regulation as well as for SCLC analysis. In addition, the cells secreted N terminal POMC, the precursor of adrenocorticotropic hormone, in response to stimulation with corticotropin releasing hormone. In summary, we established a novel cell line, SS-1 from SCLC, which produced PTHrP, PTH and N terminal POMC.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：JAPAN ENDOCRINE SOC  

The relation between the incidence of methimazole (methylmercaptoimidazole; MMI)-induced agranulocytosis and initial MMI dose was evaluated in a group of 514 patients with Graves' disease who were treated between 1995 and 2005. One hundred and forty-six (28.40%) patients had received an initial dose of 30 mg MMI and 277 (53.89%) patients had been treated with 15 mg MMI Nine patients (1.75%) developed agranulocytosis due to MMI treatment. Six (4.11 %) of 146 patients who received an initial dose of 30 mg MMI, two (4.54%) of 44 patients given an initial dose of 20 mg MMI, and one (0.36%) of 277 patients given an initial dose of 15 mg MMI developed agranulocytosis. There was a statistically significant difference in agranulocytosis incidence between patients receiving an initial dose of 30 mg MMI and those who received an initial dose of 15 mg. Although 8 (4.10%) of 195 patients in the high-dose group (20 mg or higher) developed agranulocytosis, only 1 (0.31 %) of 319 patients in the low-dose group (15 mg or lower) did. In conclusion, the incidence of agranulocytosis with low-dose MMI therapy was ten times lower than that of the high-dose regimen.

DOI： 10.1507/endocrj.K05-068

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：BIO SCIENTIFICA LTD  

Background: Two molecular forms of human GH (hGH) have beets shown to be biologically active. The 20 kDa form has been reported to have weaker diabetogenic and lipolytic actions than the 22 kDa form.
Objective: To analyze the carbohydrate metabolism of 20 kDa and 22 kDa hGH, using the adult male spontaneous dwarf rat (SDR), which is GH deficient.
Design: SDRs were given 20 kDa or 22 kDa hGH in doses of 125 mug/rat or 500 mug/rat. or saline, for 10 days, and their weight, serum IGF-I, glucose, insulin, leptin and body composition were measured.
Results: Weight and serum IGF-I increased both in the 20 kDa and 22 kDa groups, but IGF-I concentrations were significantly lower in the 20 kDa group than in the 22 kDa group. Serum glucose was not increased by either 20 kDa or 22 kDa hGH, whereas insulin was significantly increased after the higher dose of the 22 kDa hGH. Although blood concentrations of leptin were decreased by both 20 kDa and 22 kDa hGH, values were lower in the high-dose 20 kDa group than in the group given the same dose of 22 kDa hGH. Both forms of GH increased the percentage body water and body protein content, and decreased the percentage of body fat by the same degree. The observation that the higher dose of the 22 kDa hGH increased insulin concentrations without changing blood glucose demonstrates that this concentration of the hormone induces insulin resistance, whereas the same dose of 20 kDa hGH does not.
Conclusions: The results can be interpreted to indicate that the higher dose of the 22 kDa hGH has diabetogenic activity, as reported previously, whereas the 20 kDa hGH has lower diabetogenic activity. The 20 kDa form of hGH may therefore be more useful in treating adult GH deficiency, especially those with severe obesity.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer Japan  

Most gastroenteropancreatic neuroendocrine tumors contain high-affinity binding sites for somatostatin, and somatostatin-receptor scintigraphy has been introduced for the in-vivo evaluation of such tumors. We report two patients with gastroenteropancreatic neuroendocrine tumors, in whom it was quite difficult to localize the tumors by conventional techniques, and in whom we found that 111In-pentetreotide scintigraphy was useful for accurate information on tumor localization. In the first patient, who had gastrinoma, multiple tumors were shown in the gastrinoma triangle, but we could not clarify whether there were any tumors in the pancreatic body. The selective arterial secretin injection (SASI) test diagnosed that the gastroduodenal artery was the feeder of the gastrinomas, and 111In-pentetreotide scintigraphy with single-photon emission computed tomography indicated the absence of tumors in the pancreatic body. In the second patient, who had insulinoma, multiple liver tumors and a large mass in the hilum of the spleen were shown. 111In-pentetreotide scintigraphy was useful in determining that there was no secretion of insulin from the tumor in the hilum of the spleen. In conclusion, X-ray computed tomography is superior for detection of neuroendocrine tumors, because not all neuroendocrine tumors have somatostatin receptors
however, somatostatin receptor scanning, as well as the SASI test, may be useful for the surveillance of patients with known primary tumors, for monitoring patients with disseminated disease, and for following the treatment of these patients. © Springer-Verlag 2001.

DOI： 10.1007/s005340100012

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ENDOCRINE SOC  

Human GH receptor (hGHR) was recently expressed on a Ba/F3 cell line, which is a mouse pro-B cell lymphoma that has been induced to become a cloned cell line (Ba/F3-hGHR). Using a Ba/F3-hGHR cell line, we have established a bioassay for serum hGH.
hGH stimulated cell proliferation in a dose-dependent manner in concentrations ranging from 1 ng to 100 ng/mL. Cell proliferation was not influenced by other hormones or growth factors in the bioassay, with the exception of insulin-like growth factor I (IGF-I) and GH binding protein. Free IGF-I significantly stimulated the proliferation of Ba/F3-hGHR cells at concentrations over 25.85 ng/mL in this bioassay system, but serum IGF-I did not stimulate cell proliferation because the sensitivity of cell proliferation was insufficient for free IGF-I in serum. GH binding protein, however, did suppress cell proliferation at the highest concentration (100 ng/mL), but did not at the average concentration (20 ng/mL). Human serum stimulated cell proliferation, which was completely suppressed by anti-GH antibody. The GH bioactivity of serum samples from normal children and patients with non-GH deficient short stature correlated strongly with the serum hGH concentration determined by immunoradiometric assay (IRMA) (r = 0.967, r = 0.924, P < 0.0001, respectively). The ratio of bioactivity/IRMA was 1.01 +/- 0.26 in sera from normal children and 1.18 +/- 0.24 and 1.00 +/- 0.29 at basal values and peak values in GH stimulation tests, respectively, in sera from patients with non-GH deficient short stature. The bioactivity/IRMA ratio for the serum GH bioactivity of a patient who had biologically inactive GH caused by an amino acid substitution was 0.333 +/- 0.056 (mean +/- SD).
In conclusion, we established a new sensitive bioassay for hGH that is specific for hGH somatogenic action and is useful for screening of patients with short stature caused by biologically inactive hGH.

DOI： 10.1210/jcem.85.11.6983

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記述言語：日本語   出版者・発行元：(株)自然科学社  

症例は59歳女性で頸部腫瘤を自覚した.甲状腺機能は正常,MCPAが陽性,甲状腺超音波で甲状腺左葉の腺腫様結節で気管変位があり摘出術を受けた.その後,飲酒後に気分が悪くなり甲状腺機能亢進症を指摘された.超音波検査の結果よりバセドウ病と診断し,MMIを投与,1ヵ月後にFTを投与し改善した.症例2は62歳女性で超音波下吸引細胞診で微小乳癌と診断された.甲状腺機能は正常でMCPAとTGHA陽性であった.左葉摘出術を受けた.腰椎すべり症手術で入院したところ,手指振戦と頻脈を指摘された超音波検査結果よりバセドウ病と診断した.甲状腺術後のバセドウ病の発症迄の期間はまちまちである.原因として甲状腺切除により大量の障害された甲状腺上皮細胞が血中に放出され抗原提示された可能性も考えられる

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：CHURCHILL LIVINGSTONE  

The somatogenic action of the 20 kilodalton human growth hormone (20 K) was studied using the spontaneous dwarf rat (SDR), which has an isolated GH deficiency. Saline or 2.5 mug, 10 mug, or 100 mug/rat/day of recombinant 20 K or 22 K was administered to prepubertal male and female SDRs for 10 days. Their body weights, serum IGF-I, glucose and insulin were measured, and their body composition was determined. Body weights and serum IGF-I increased dose-dependently in both the 20 K- and 22 K-treated groups. There was no significant difference in body weights and serum IGF-I between the 20 K- and 22 K-treated groups except at the dose of 100 mug/rat, in which the IGF-I concentrations were higher in the 22 K-treated male SDRs (P < 0.05: 20 K vs 22 K). Blood glucose was not significantly different between the Spague-Dawley (SD) normal rats and the SDR control groups; however, serum insulin levels of the SDR were higher than those of the SD control group (P < 0.05). Additionally, there was a tendency for serum insulin and glucose levels to increase following 22 K treatment, but the differences were not significant. The percentage of body fat decreased with hGH treatment in both groups (P < 0.01: GH 10, 100 <mu>g/rat group vs SDR control group), however, no significant differences were observed in body composition between the 20 K and 22 K treatment groups.
In summary, the 20 K-hGH showed almost the same somatogenic activity as the 22 K-hGH in prepubertal male and female SDRs. (C) 2000 Harcourt Publishers Ltd.

DOI： 10.1054/ghir.2000.0153

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ENDOCRINE SOC  

Twenty-kilodalton human GH (20K), which is one of the human GH (hGH) variants, is thought to be produced by alternative premessenger ribonucleic acid splicing. However, its physiological role is still unclear due to the lack of a specific assay. We have measured serum 20K and 22-kDa hGH (22K) by specific ELISAs to investigate the physiological role of 20K in children.
The subjects were 162 normal children, aged 1 month to 20 yr; 12 patients with GH deficiency (GHD), aged 11 months to 13 yr; 57 children with non-GHD short stature, aged 2-17 yr; and 13 girls with Turner's syndrome, aged 5 months to 15 yr. Samples were collected at random from normal children and were collected after hGH provocative tests and 3-h nocturnal sleep from GHD, non-GHD short stature, and Turner's syndrome children. The mean basal serum concentrations of 22K and 20K were 2.4 +/- 2.8 ng/mL and 152.3 +/- 184.0 pg/mL in normal bays and 2.5 +/- 3.1 ng/mL and 130.6 +/- 171.5 pg/mL in normal girls, respectively, The percentages of 20K (%20K) were 5.8 +/- 2.1% and 6.0 +/- 3.2% in 83 normal boys and 79 normal girls, respectively. There was no significant difference in %20K either among ages or between the prepubertal stage and the pubertal stage in normal boys and girls. The mean %20K values in basal samples of provocative tests in 12 patients with GHD, non-GHD short stature, and Turner's syndrome were 6.5 +/- 2.4%, 6.5 +/- 3.8%, and 5.9 +/- 3.2%, respectively. There was no significant difference in %20K among normal children and these growth disorders, and there was no significant difference in %20K throughout the hGH provocative tests and 3-h nocturnal sleep in these growth disorders. There was also no significant correlation elation between the percentage of 20K and the height so score or body mass index in either normal children or subjects with these growth disorders.
In conclusion, the %20K is constant, regardless of age, sex, puberty, height so score, body mass index, and GH secretion status. The regulation of serum 20K levels remains to be established.

DOI： 10.1210/jcem.84.1.5402

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記述言語：日本語   出版者・発行元：日本内科学会-関東地方会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本病態栄養学会  

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記述言語：日本語   出版者・発行元：(一社)日本病態栄養学会  

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記述言語：日本語   出版者・発行元：日本内科学会-関東地方会  

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記述言語：日本語   出版者・発行元：(一社)日本神経学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(NPO)日本呼吸器内視鏡学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病・妊娠学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病・妊娠学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：日本内科学会-関東地方会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病・妊娠学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病・妊娠学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

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記述言語：日本語   出版者・発行元：日本内科学会-関東地方会  

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記述言語：日本語   出版者・発行元：(公財)成長科学協会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本糖尿病学会  

症例は28歳女性。10歳時に頭蓋咽頭腫摘出術後に汎下垂体機能低下症となり、13歳まで成長ホルモン(以下GHと略す)の補充療法を、また現在まで継続して副腎皮質、甲状腺、抗利尿ホルモンの補充療法を受けている。手術後より体重が増加し、13歳時BMI26.5から16歳時にBMI29.6と肥満が増悪し、糖尿病と診断されインスリン治療が開始された。今回は28歳時に下痢発症後四肢脱力を呈し救急搬送され、高ナトリウム血症および低カリウム血症が認められた。下痢とデスモプレシンの点鼻失念による脱水と電解質異常を来した病態と診断された。脱力に関してはヒドロコルチゾンの内服失念による副腎不全と低カリウム血症の関与が疑われ、緊急入院となった。補液とデスモプレシン点鼻により高ナトリウム血症は改善し、副腎不全に対しては最終的にヒドロコルチゾン内服25mg/日で退院となった。一方、糖尿病は食事療法の遵守により血糖コントロールが改善し、第17病日でインスリンは中止となったが、早朝空腹時の低血糖を来すようになった。アルギニン負荷試験でGH分泌が低反応であり、成人成長ホルモン分泌不全(以下AGHDと略す)と診断した。低血糖の原因として、夜間のGH分泌不全による肝臓の糖新生の低下が考えられた。インスリノーマや副腎ホルモンの補充不足は否定的であった。低血糖の程度は軽く、眠前に80Kcalの補食摂取により空腹時血糖60mg/dl前後の維持することができ、GHの補充療法は行わず退院とした。糖尿病を併発したAGHDの患者で、早朝低血糖を頻発し空腹時血糖の決定因子としてGHの重要性を示唆する症例を経験したので報告する。(著者抄録)

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：東邦大学医学会  

CiNii Books

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記述言語：日本語   出版者・発行元：東邦大学医学会  

Sulfonylurea(SU)薬の1種であるグリメピリドは、その副作用に低血糖がある。肝臓で代謝され、腎臓から排泄されるグリメピリドは、肝障害や腎障害があると低血糖が重篤化しやすいことが知られている。グリメピリドを内服中に低血糖とそれによる意識障害にて、救急搬送された本症例は、入院後、低血糖が遷延した。肝障害や腎障害はなく、低血糖の原因検索の結果、非機能性下垂体腺腫と下垂体前葉ホルモンのうち成長ホルモン(growth hormone:GH)と黄体刺激ホルモン(luteinizing hormone:LH)、卵胞刺激ホルモン(follicle stimulating hormone:FSH)の分泌不全のみを認めた。この中でGHは低血糖時のカウンターホルモンとして知られており、低血糖の遷延はGH分泌不全によるものと考えた。低血糖の原因には薬剤性以外にも様々な要因がある。糖尿病患者において低血糖が遷延した場合には、本症例のように他疾患が隠れている場合もあり、他の低血糖を引き起こす原因検索を行うべきであると考え、本症例を報告する。(著者抄録)

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その他リンク： http://search.jamas.or.jp/link/ui/2010290977

記述言語：日本語   出版者・発行元：日本内科学会-関東地方会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：英語   掲載種別：研究発表ペーパー・要旨（国際会議）   出版者・発行元：JAPAN ENDOCRINE SOC  

Web of Science

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記述言語：日本語  

J-GLOBAL

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記述言語：英語   掲載種別：速報，短報，研究ノート等（学術雑誌）   出版者・発行元：MARY ANN LIEBERT, INC  

DOI： 10.1089/thy.2008.0192

Web of Science

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記述言語：日本語  

J-GLOBAL

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記述言語：日本語   出版者・発行元：日本内科学会-関東地方会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：東邦大学医学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：東邦大学  

CiNii Books

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記述言語：日本語   出版者・発行元：東邦大学医学会  

CiNii Books

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記述言語：日本語   出版者・発行元：東邦大学  

CiNii Books

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記述言語：日本語   出版者・発行元：(株)日本医事新報社  

クッシング症候群のほとんどは副腎腺腫によるものである.その他の稀な病態には,原発性副腎皮質結節性異形成,異所性コルチゾール産生腫瘍,異所性CRH産生腫瘍などがある.これら,どの病態においても,早期診断をするには,一般検査所見や特徴的な症状から本症候群の存在を推測し,内分泌検査を行う必要性がある.そこで,症状ならびに一般検査所見から,診断に必要な内分泌学的検査や画像検査について述べた

CiNii Books

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

76歳男.主訴は左側腹部痛.左頸部リンパ腫大を認め,また,腹部CTで右副腎の腫大及び腹腔内リンパ節多数の腫大が認められた.身体所見,一般検査所見,画像所見,頸部リンパ節生検より,most probable diseaseはparagangliomaと考えられた.血中・尿中コルチゾール値は正常範囲内であったが,Na低値・K高値であったため,rapid ACTH試験を施行した.その結果,コルチゾールの前値は高値ではなかったが,ACTH負荷後も上昇を認めなかった.腹部CT上も右副腎は腫大し左副腎はリンパ節と一塊となっていることから,腫瘍の浸潤による副腎不全と判断し,ソルコーテフ50mg/dayの補充を開始した.以後Na・K値共に正常範囲内への回復が認められた.また,入院時は腹部に腫瘤を触知しなかったが,経過中に触知するにいたった.悪性腫瘍に対しては化学療法を開始したが,1クール目開始後day7で全身状態が悪化し永眠された

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記述言語：日本語  

CiNii Books

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記述言語：日本語   出版者・発行元：東邦大学医学会  

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記述言語：英語  

Twenty-kilodalton human GH, a human GH (hGH) variant, has insulin-like and anti-insulin-like actions different from 22-kilodalton hGH (22K). The diabetogenic action of twenty-kilodalton hGH (20K) is reported to be weaker than that of 22K, yet it is still unclear whether secretion of 20K is influenced by factors in carbohydrate metabolism. We measured serum 20K and 22K in children with simple obesity and studied the regulation of 20K production. The subject were 124 boys (9.78±1.94 yr old, body mass index (BMI) 25.88±2.43 kg/m^2) and 54 girls (10.29±2.25 yr old, BMI 26.90±3.72 kg/m^2) with simple obesity. The serum samples were taken after overnight fasting. The data was compared with the data from children with non-GH deficient (GHD) short stature. The mean serum concentrations of 22K and 20K were 1.47±1.63 ng/ml and 81.3±80.3 pg/ml in obese boys and 1.86±2.00 ng/ml and 108.9±120.4 pg/ml in obese girls. The percentage of 20K in hGH was 7.4±5.0% in obese boys and 6.5±2.9% in obese girls, and the percentage was not significantly different from the percentage of 20K in children with non-GHD short stature. The height SD score, obesity index, body fat mass, BMI, fasting blood sugar, insulin, homeostasis model assessment-R (HOMA-R), HbA1c, total cholesterol, triglyceride, leptin and IGFBP-1 were not correlated to the percentage of 20K. We conclude that the percentage of 20K is constant not only in normal children but also in obese children, and factors that correlate with obesity such as glucose and insulin do not influence the 20K splicing mechanism.

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その他リンク： http://search.jamas.or.jp/link/ui/2004095633

記述言語：日本語  

CiNii Books

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(有)医学の世界社  

確定診断された副腎腫瘍20例を対象に末梢血中のステロイドホルモンを同時一斉分析してステロイドホルモン合成酵素活性を比較検討した.その結果,非機能性腺腫4例では血中dehydroepindrosterone(DHEA)とΔ4androstenedione(Δ4A)が正常群に比べて有意に低値を示した.そこで非機能性腺腫を正常群と低値群に分け,これにpreclinical Cushing症候群を加えてホルモン合成酵素活性を検討した.その結果非機能性腺腫低値群とpreclinical Cushing症候群では17,20-lyase活性の低下がみられた.その他のステロテドホルモン合成酵素活性は有意差がみられなかった.このことから非機能性腺腫の一部は将来的にpreclinical Cushing症候群に移行する可能性も考えられた

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語  

CiNii Books

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(有)医学の世界社  

症例は70歳男で,約2年前,他院のスクリーニング検査で多発性肝腫瘍を指摘され,精査目的に自施設を受診した.各種検査により神経内分泌癌の肝転移と診断したが,原発巣は不明であった.この時血中インスリン値は正常で,低血糖症状もみられなかった.化学療法により肝腫瘍は増大することなく経過したが,1ヵ月前頃より早朝空腹時に失見当識,意識混濁がみられるようになった.今回,肝腫瘍の経過観察目的に腹部超音波検査を行うため昼食を取らずに来院したところ,意識が朦朧とし見当識障害や冷汗がみられたため緊急入院した.臨床症状や随時血糖が著明に低値であることなどから血中インスリンを測定したところ高インスリン血症を認め,インスリノーマと診断した.腹部超音波検査では肝内に多数の低エコー域を認め,これらはインスリノーマの転移巣であることが判明した

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記述言語：日本語   出版者・発行元：(公財)成長科学協会  

血清サンプルは23名の正常小児(男児14名,女児9名),成長ホルモン分泌不全のない低身長小児10名(男児6名,女児4名)と,生物学的不活性成長ホルモンによる低身長小児(1)から得た.ヒト成長ホルモンは用量依存性にBa/F3-hGHR細胞株の増殖を促進した.ヒト成長ホルモンのBa/F3-hGHR細胞株の増殖促進作用は抗ヒト成長ホルモン抗体により完全に抑制された.抗成長ホルモン抗体は血清サンプルのBa/F3-hGHR細胞株増殖促進活性を完全に抑制した.正常小児血清中の成長ホルモンのbioactivityとimmunoreactivityは有意な正の相関を示した.成長ホルモン分泌不全のない低身長小児血清中の成長ホルモンのbioactivityとimmunoreactivityも有意な正の相関を示した.以上より,生物学的不活性成長ホルモンによる成長障害の頻度は高くないと考えられた

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(株)日本臨床社  

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記述言語：日本語   出版者・発行元：東邦大学医学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語   出版者・発行元：(一社)日本内分泌学会  

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記述言語：日本語  

CiNii Books

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