Language：English   Publishing type：Research paper (scientific journal)  

Mitochondrial dynamics-related RNAs during hospitalization for acute heart failure (AHF) were rarely evaluated in various points. In total, 234 patients who visited the emergency room for AHF were retrospectively evaluated. Blood samples were collected within 15 min of admission (day 1), after 48-72 h, and between days 7 and 21. Low miR-140-3p during hospitalization was defined as the level being categorized as Q1 more than once (on days 1, 3 and/or 14), and normal-140-3p during hospitalization as the level never being categorized as Q1. The median miR-140-3p levels were significantly decreased on days 3 and 14 (2.53 [1.06-6.42] and 3.65 [1.41-9.05], respectively) in comparison to the value on day 1 (6.71 [2.66-14.0]). Kaplan-Meier curves indicated that all-cause mortality within 1000 days was significantly higher in the low-miR-140-3p group than in the other-miR-140-3p group on days 1 and 14. Moreover, the survival rate was significantly lower and the rate of HF events was significantly higher in the low-miR-140-3p group than in the normal-miR-140-3p group. The miRNA levels of patients further decreased during treatment for AHF. Low levels of mitochondrial fission-related miRNAs during AHF treatment were independently associated with an increased risk of long-term adverse outcomes.

DOI： 10.1038/s41598-025-23792-4

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

Mitochondria are dynamic organelles that can change their morphology. The role of these mitochondrial dynamics in cardiomyocytes remains obscure in patients with heart failure (HF). Endomyocardial biopsies were performed consecutively in 127 HF patients, and mitochondrial morphology data were obtained from 111 patients by electron microscopy. The patients were divided into three groups according to mitochondrial area quartiles (fission [Q1, area ≤ 0.119 μm2, n = 27], normal [Q2/Q3, 0.120 μm2 ≤ area ≤ 0.178 μm2, n = 55], and fusion [Q4, area ≥ 0.179 μm2, n = 28]). In the fission group, the serum N-terminal pro-brain natriuretic peptide and B-type natriuretic peptide (BNP) levels were significantly higher, and patients with HF and a reduced left ventricular ejection fraction were more common, than in the other groups. A multivariate logistic regression model showed that diabetes mellitus was independently associated with placement in the fission group (odds ratio: 2.835, 95%confidence interval [CI]: 1.037-7.752). A Kaplan-Meier curve analysis showed that the prognosis was significantly poorer in the fission group than in the other groups, and a multivariate Cox regression model revealed fission to be an independent predictor of 1500-day mortality (hazard ratio: 4.365, 95%CI: 1.198-15.909). The circulating levels of miR-140-5p (≥2500) were independently associated with the presence of mitochondrial fission (OR: 3.622, 95%CI: 1.260-10.413). Excessive mitochondrial fission was observed in patients with severe HF status, and was independently associated with adverse outcomes in HF patients. Circulating mitochondrial dynamics-related miRNA levels might be of use in detecting mitochondrial fission in the cardiomyocytes of HF patients.

DOI： 10.1038/s41440-025-02338-1

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: This study aimed to elucidate the age trends among non-surgical patients requiring intensive care over a 10-year period and the prognostic impact of aging in relation to their underlying etiologies. METHODS AND RESULTS: In all, 4,279 non-surgical patients requiring intensive care from 2012 to 2021 were enrolled in the study. Patient backgrounds and prognoses were compared among age 4 groups: Group A, age <60 years (n=910); Group B, age 60-69 years (n=1,062); Group C, age 70-79 years (n=1,355); and Group D, age ≥80 years (n=952). During the study period, the number of patients aged 60-69 years decreased significantly with time, whereas the number aged over 80 years increased significantly. A multivariate Cox regression model identified Group D as an independent predictor of 365-day all-cause mortality (hazard ratio [HR] 2.070; 95% confidence interval [CI] 1.619-2.646) relative to Group A. Multivariate logistic regression analysis indicated that the presence of sepsis was independently associated with 365-day mortality, especially in the cohort aged ≥80 years (HR 1.878; 95% CI 1.270-2.777; P=0.002). CONCLUSIONS: The mean age of patients requiring non-surgical intensive care is increasing annually, and greater age was identified as a significant factor associated with a higher 365-day mortality rate. The presence of sepsis was linked to increased 365-day mortality among older individuals.

DOI： 10.1253/circj.CJ-24-1030

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: Mitochondria are dynamic organelles whose morphology can change through fusion and fission. The role of these mitochondrial dynamics in cardiomyocytes has not been evaluated in patients with acute heart failure (AHF). METHODS AND RESULTS: Two hundred seventy-three AHF patients and 374 control patients who attended a cardiovascular outpatient clinic were prospectively analyzed. Blood samples were collected within 15 min of admission from AHF patients (AHF group) and control patients who visited a daily cardiovascular outpatient clinic (control group). Mitochondrial dynamics-related RNAs were compared between the two groups, and long-term prognosis (1000-day mortality) was evaluated across RNA quartiles. The miR-140-3p and miR-485-3p levels in the AHF group (median 6.73, range 2.75-14.55; median 0.45, range 0.13-1.86) were significantly (p = 0.002 and p = 0.049) lower than those in the control group (median 8.61, range 3.37-34.12; median 0.78, range 0.15-3.52), respectively. Cardiac biomarkers, including N-terminal pro-BNP, heart-type fatty acid-binding protein, and high-sensitivity troponin, were significantly higher in the low-miR-140-3p group than in the middle- and high-miR-140-3p groups. Kaplan-Meier curve analysis revealed that all-cause mortality within 1000 days was significantly higher in the low-140-3p group than in the middle- and high-miR-140-3p groups. Cox regression analysis revealed that the low-140-3p group was an independent predictor of 1000-day mortality (hazard ratio 1.567; 95 % confidence interval 1.004-2.444; p = 0.048). CONCLUSION: Impaired mitochondrial dynamics were observed in the acute phase of AHF. In particular, a mitochondrial fission-related miRNA (miR-140-3p) was independently associated with adverse outcomes.

DOI： 10.1016/j.ijcard.2025.133967

PubMed

researchmap

Language：English   Publishing type：Research paper (scientific journal)  

BACKGROUND: The association between the clinical significance and prognostic impact of catheter-based micro-axial ventricular assist device Impella (Abiomed, Danvers, MA, USA) support and age has not been sufficiently investigated. METHODS AND RESULTS: A total of 5282 patients treated with Impella were analyzed from the Japanese registry for Percutaneous Ventricular Assist Device (J-PVAD). Patients were categorized into four age groups: <50 years old (Group A, n = 670), 50-69 years old (Group B, n = 2009), 70-79 years old (Group C, n = 1716), and ≥80 years old (Group D, n = 887). Kaplan-Meier curve analysis demonstrated that the prognosis, including 30-day all-cause mortality, was significantly poorer in Group D compared to the other groups. A multivariate Cox regression model identified Group D as an independent predictor of 30-day all-cause death (HR: 2.173, 95 % CI: 1.699-2.779) relative to Group A. The multivariate logistic regression analysis indicated that estimated glomerular filtration rate, lactate levels, and systolic blood pressure were independently associated with 30-day mortality across all age categories. The presence of more than two major adverse events was independently associated with 30-day mortality specifically in the cohort aged ≥80 years (OR: 1.492, 95 % CI: 1.037-2.148, p = 0.031). CONCLUSION: Increasing age was identified as a significant factor associated with higher 30-day mortality rates in J-PVAD. While the incidence of major adverse events did not differ significantly across age categories, the occurrence of two or more additional adverse events was linked to increased 30-day mortality among older individuals. Therefore, an appropriate mechanical support strategy may be necessary for elderly Japanese patients based on their age.

DOI： 10.1016/j.jjcc.2024.11.007

PubMed

researchmap

Language：English   Publisher：(一社)日本循環器学会  

researchmap

J-GLOBAL

researchmap

J-GLOBAL

researchmap

Language：Japanese   Publisher：(株)日本医事新報社  

researchmap

Language：English   Publisher：(一社)日本心血管インターベンション治療学会  

researchmap