Language：English   Publishing type：Research paper (scientific journal)  

Cholinergic signaling in the retina is mediated by acetylcholine (ACh) released from starburst amacrine cells (SACs), which are key neurons for motion detection. SACs comprise ON and OFF subtypes, which morphologically show mirror symmetry to each other. Although many physiological studies on SACs have targeted ON cells only, the synaptic computation of ON and OFF SACs is assumed to be similar. Recent studies demonstrated that gene expression patterns and receptor types differed between ON and OFF SACs, suggesting differences in their functions. Here, we compared cholinergic signaling pathways between ON and OFF SACs in the mouse retina using the patch clamp technique. The application of ACh increased GABAergic feedback, observed as postsynaptic currents to SACs, in both ON and OFF SACs; however, the mode of GABAergic feedback differed. Nicotinic receptors mediated GABAergic feedback in both ON and OFF SACs, while muscarinic receptors mediated GABAergic feedback in ON SACs only in adults. Neither tetrodotoxin, which blocked action potentials, nor LY354740, which blocked neurotransmitter release from SACs, eliminated ACh-induced GABAergic feedback in SACs. These results suggest that ACh-induced GABAergic feedback in ON and OFF SACs is regulated by different feedback mechanisms in adults and mediated by non-spiking amacrine cells other than SACs.

DOI： 10.1085/jgp.202413550

PubMed

researchmap

Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1096/fasebj.2021.35.s1.01591

researchmap

Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/jnc.15217

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATURE PUBLISHING GROUP  

Synchronized oscillatory activity is generated among visual neurons in a manner that depends on certain key features of visual stimulation. Although this activity may be important for perceptual integration, its functional significance has yet to be explained. Here we find a very strong correlation between synchronized oscillatory activity in a class of frog retinal ganglion cells ( dimming detectors) and a well-known escape response, as shown by behavioral tests and multi-electrode recordings from isolated retinas. Escape behavior elicited by an expanding dark spot was suppressed and potentiated by intraocular injection of GABA(A) receptor and GABA(C) receptor antagonists, respectively. Changes in escape behavior correlated with antagonist-evoked changes in synchronized oscillatory activity but not with changes in the discharge rate of dimming detectors. These antagonists did not affect the expanding dark spot - induced responses in retinal ganglion cells other than dimming detectors. Thus, synchronized oscillations in the retina are likely to encode escape-related information in frogs.

DOI： 10.1038/nn1497

Web of Science

researchmap

Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Japanese Psychonomic Society  

In the retinas of frogs synchronized discharges accompanied by γ-range oscillations (the oscillatory synchronization, OS) are generated among OFF-sustained ganglion cells (dimming detectors) in response to a dimming stimulus. We have already shown that an expanding dark spot generates the retinal OS and elicits escape behavior, which is abolished when the OS is suppressed by a GABA_A receptor antagonist. Recently we observed that a GABA_C receptor antagonist, (1,2,5,6-tetrahydropyridine-4-yl) methylphosphinic acid (TPMPA), enhanced the oscillatory activity of dimming detectors. In the present study, we investigated how OS and escape behavior were modulated when TPMPA was applied to the retina. An intraocular injection of TPMPA potentiated escape behavior. Moreover, multi-electrode recordings from isolated retinas revealed that the OS was significantly enhanced by TPMPA. On the other hand, TPMPA did not affect the response properties of other ganglion cells which were not dimming detectors. Furthermore, modulation of the retinal OS by the GABA receptor antagonists was closely related to the changes in escape behavior. Consequently, we conclude that the OS may encode escape-related information.

DOI： 10.14947/psychono.KJ00004348788

researchmap

researchmap

J-GLOBAL

researchmap

Language：English   Publishing type：Research paper, summary (international conference)   Publisher：ELSEVIER IRELAND LTD  

DOI： 10.1016/j.neures.2009.09.1123

Web of Science

researchmap

Language：English   Publishing type：Research paper, summary (international conference)   Publisher：SPRINGER TOKYO  

Web of Science

researchmap

Language：English   Publishing type：Research paper, summary (international conference)   Publisher：ELSEVIER IRELAND LTD  

Web of Science

researchmap

Language：Japanese   Publisher：日本基礎心理学会  

DOI： 10.14947/psychono.KJ00004414561

researchmap

researchmap

researchmap

researchmap