Language：Japanese   Publisher：一般社団法人 日本老年歯科医学会  

Purpose The aim of this study wasto clarify the efficacy of the management of oral health care carried out by dental hygienists at a nursing home.<BR>Methods: A total of 142 subjects, living in twofacilities (facility A; 82 subjects, mean age 85.9±7.5 years, and facility B; 60 subjects, mean age 85.6±8.3 years) for dependent elderly persons in Yamanashi Prefecture, participated in this study. The intervention period was 8 months, and the evaluation of oral hygiene conditions by visual examination and oral bacteria test was performed three times: before intervention, after 4 months, and after the end of the intervention. In facility A, dental hygienists performed general oral cleaning for 20 randomly-chosen subjects directly, and gave advice aboutoral health care to the other subjects. In facility B, dental hygienists prepared an interventional oral care plan for the subjects, based on the risk assessment of each subject's oral condition, and conducted oral care intervention in cooperation with the facility staff. In addition, a survey of the attitude of nursing stuff toward the interventional plan was conducted.<BR>Results: In subjects at facility A, there was no significant difference in the number of oral bacteria within the intervention period, while in subjects at facility B, the number of oral bacteria at the last intervention significantly decreased (p<0.05). The results of the survey indicated some differences between the two facilities in the attitude of the facility staff to the interventional plan.<BR>Conclusion: Management of oral health care at a nursing home was proven to be mo t effective when a total oral health care plan was made, considering the facilities, equipment and the dental hygienists' interventional method. We confirmed the importance of the management of oral health care.

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Language：Japanese   Publisher：一般社団法人 日本老年歯科医学会  

Oral bacteria counts are used as an assessment index for oral health care. In this study, we examine the effect of pressure applied to the swab in order to standardize the collection of swab samples.<BR>Samples were collected from the tongues of 35 healthy adults. The swabs were attached to a device to achieve sample collection pressures of 5, 20 and 40 g. Bacterial counts were made on each swab by a bacteria counter using the dielectrophoretic impedance measurement method, and compared.<BR>The results showed that higher bacterial counts were obtained with higher sample collection pressures (p<0.01); further, bacterial counts were significantly different for swabs taken at sampling pressures of 5 and 20 g and 5 and 40 g (p<0.01). As variations in sampling pressure applied to the tongue are inherent in manual swabbing, it is necessary to standardize sample collection pressure for measuring oral bacterial counts as an assessment index. Considering the stability of swabbing and the patients' comfort, pressure of 20 g is suitable for sampling from the tongue.

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Language：Japanese   Publisher：特定非営利活動法人 日本歯科保存学会  

We evaluated the effect of dentifrice containing 3% vitamin C on periodontitis. Institutional ethics committee approval was obtained prior to the study. Sixty patients (26 men and 34 women) with periodontitis were enrolled in this double-blind clinical trial. We prepared three types of dentifrice: one containing 5.0% zeolite, 0.68% sodium monofluorophosphate, 0.05% cetylpyridinium chloride and 0.05% β-glycyrrhetinic acid (D-1); one with 1.0% vitamin C added to D-1 (D-2); and one with 3.0% vitamin C added to D-1 (D-3). Dentifrice was randomly assigned among subjects, each of whom was required to brush twice daily for 4 weeks. Periodontal indices were measured at baseline and at 2- and 4-week evaluations. The results for mean gingival index (GI), probing depth (PD) and bleeding scores in all groups revealed statistically significant differences between at baseline and at the 2-week evaluation (p<0.01) and between at baseline and at the 4-week evaluation (p<0.01). The results for mean GI, PD and bleeding scores with D-2 and D-3 revealed statistically significant differences between D-1 and D-2 and between D-1 and D-3 at the 2- and 4-week evaluations (p<0.01). Of the three dentifrices, D-3 was the most effective. Statistically significant differences in mean GI (p<0.05) and PD (p<0.05) were observed between D-2 and D-3. These results suggest that a dentifrice containing 3% vitamin C is more effective against periodontitis than one containing 1% vitamin C.

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Language：Japanese   Publisher：一般社団法人 日本老年歯科医学会  

In the present study, the relationships between the counts of putative periodontopathic bacteria in saliva and theoral and general status of the elderly in nursing homes were investigated. The subjects were 37 residents (13 men and 24 women; mean age, 82.1 years) of six nursing homes. Saliva samples were collected from subjects, who providedconsent. Bacterial counts per 10, μl of saliva were measured using the PCR-Invader method for the following species: <I>Aggregatibacter actinomycetemcomitans (A. a), Prevotella intermedia (P. i), Porphyromonas gingivalis (P. g), Tannerella forsythia (T.f) </I>, and <I>Treponema denticola (T.d) </I>. The relationships between the measured bacterial countsand the following subject characteristics were investigated: age, gender, care level, BMI, periodontal parameters, amount of tongue coating, and amount of food residue. The severe periodontal disease group had significantly highercounts of <I>P.g, T.f</I>, and <I>T. d</I> than the mild or moderate group (p<0.05, p<0.05, and p<0.01, respectively), while nosignificant relationships to bacterial counts were observed for care level, BMI, and amount of food residue. Theseresults suggest that the periodontopathic bacteria detected in saliva are primarily related to the severity ofperiodontitis of the elderly and are independent of their general status.

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Language：Japanese   Publisher：(NPO)日本歯周病学会  

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Language：Japanese   Publisher：特定非営利活動法人 日本歯周病学会  

We evaluated the effect of dentifrice containing sodium ascorbate on periodontitis. Institutional ethics committee approval was obtained prior to the study. Subjects were 53 patients (15 men and 38 wemen, average age : 44.0 years) with periodontits enrolled in a double-blind clinical trial. We prepared 4 types of dentifrice, i.e., that containing zeolite and sodium monofluorophosphate (D-1), that adding 1.0% sodium ascorbate to D-1 (D-2), that adding 0.1% dl-α-tocopheryl acetate, 0.05% cetylpyridimium chloride, and 0.05% β-glycyrrhetinic acid to D-1 (D-3), and that adding 1.0% sodium ascorbate to D-3 (D-4). Dentifrice was randomly assigned to subjects, who were asked to brush twice daily for 4 weeks. Periodontal indices were measured at baseline, 2-, and 4-week evaluation. As a result for mean bleeding scores of D-2 and D-4, the dentifrices containing sodium ascorbate, a statistically significant difference was seen between baseline and 4-week evaluation (p<0.05, p<0.01). Of the 4 dentifrices, D-4 was the most effective. Statistically significant differences in mean gingival index (p<0.01), probing depth (p<0.01), and bleeding score (p<0.01) were seen between baseline and 4-week evaluation. These results suggest that a dentifrice containing sodium ascorbate is effective against periodontitis.

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Language：Japanese   Publisher：(NPO)日本歯周病学会  

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Language：Japanese  

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Language：Japanese   Publisher：特定非営利活動法人日本歯周病学会  

We studied risk indicators in saliva for periodontal disease in the elderly. Thirty-six subjects over 65 years old (15 men and 21 women) were divided into 4 groups based on clinical evaluation: 7 with healthy gingiva or gingivitis, 11 with moderate periodontitis, 9 with severe periodontitis, and 9 edentulous subjects. Whole saliva samples were analyzed for the presence of <I>Actinobacillus actinomycetemcomitans</I>, <I>Porphyromonas gingivalis</I>, <I>Prevotella intermedia</I>, <I>Bacteroides forsythus</I>, and <I>Treponema denticola</I> using polymerase chain reaction (PCR) method. We studied the relationship of periodontal parameters to the presence of these periodontopathic bacteria, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and interleukin-1 (IL-1) gene polymorphism. The prevalence of <I>Bacteroides forsythus</I> in subjects with severe periodontitis was significantly higher than in those with healthy gingiva or gingivitis (p<0.05). LDH activity increased proportionally with the advance of periodontitis (p<0.05). AST and IL-1 polymorphism did not correlate significantly with periodontal status. This suggests that the presence of Bacteroides forsythus and LDH activity in whole saliva are possible to be risk indicators for periodontal disease in the elderly. J Jpn Soc Periodontol 44 : 261-272, 2002.

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Language：Japanese  

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Language：Japanese   Publisher：日本医療機器学会  

It is well known that there are many substances related to the diseases at very low concentration in human body fluid. An attempt to search the new marked substances in human body fluid related the prediction of diseases has been carried out by high sensitive and simple detetecting method. In this report, the reactivities of fresh human saliva were examined by the peroxidase activity (POD) and the diminishing of the specific color (at 515 nm) of diphenylpicrylhydrazyl (DPPH)/MeOH solution used as the marker in addition of saliva. It was observed that the POD was depened on the progressing of dental caries, and that the behavior of DPPH was not similar to that of POD. Then, the color diminishing of DPPH was monotonically decresed according with the POD increase. It is expected thet this examinating method will be useful to the prodiction and the prevention of the diseases.

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Language：Japanese  

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